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LC Analysis of JS38, a Novel Antineoplastic Medicine, in the Plasma, Urine, Bile, Feces, and Other Tissues of Rats

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Abstract

An RP-LC method has been developed for analysis of JS38 in the plasma, urine, bile, feces, and important tissues of rats. Chromatography was performed on a C18 analytical column with 80:20 (%, v/v) acetonitrile–phosphate buffer (0.5% phosphoric acid and 0.3% TEA adjusted to pH 5.0) as mobile phase at a flow-rate of 1.0 mL min−1. Eluted compounds were detected at 400 nm by ultraviolet diode-array detection (DAD). The method was validated for linearity, accuracy (recovery from biological matrixes such as plasma, urine, bile, feces, and important organs), repeatability (within-day and between-day precision), and stability. The results indicate that the method is suitable for pre-clinical pharmacokinetic study of JS38.

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Authors and Affiliations

  1. Tongji University, Tongji Hospital, 389 Xincun Road, Shanghai City, China

    Hong Zhang, Yu Fang & Ying Li

Authors
  1. Hong Zhang
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  2. Yu Fang
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  3. Ying Li
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Correspondence to Hong Zhang or Yu Fang.

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Zhang, H., Fang, Y. & Li, Y. LC Analysis of JS38, a Novel Antineoplastic Medicine, in the Plasma, Urine, Bile, Feces, and Other Tissues of Rats. Chroma 68, 409–414 (2008). https://doi.org/10.1365/s10337-008-0736-x

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  • DOI: https://doi.org/10.1365/s10337-008-0736-x

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