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UPLC–MS–MS Analysis of Baicalin in the Cerebrospinal Fluid of Rabbits: Application to a Pharmacokinetic Study

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Abstract

A sensitive and selective ultra-performance liquid chromatographic–tandem mass spectrometric method has been developed for analysis of baicalin in the cerebrospinal fluid of rabbits. Samples were separated on a C18 column with a gradient prepared from acetonitrile and 0.3% aqueous formic acid solution as mobile phase. The target compounds were quantified by multiple reaction monitoring (MRM) using electrospray ionization (ESI). Intra-and inter-day accuracy, precision, and linear range were investigated in detail. The lower limit quantification (LOQ) was 0.108 ng mL−1. The method has been successfully used for evaluation of the pharmacokinetics of baicalin in 12 rabbits.

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Acknowledgments

This work was supported by the Science and Technology Program of Hunan Province (05SK3002) of China, the National Natural Science Foundation of China (Grant No. 20475066), and the International Cooperation Project on Traditional Chinese Medicines of the Ministry of Science and Technology of China (2006DFA04090).

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Authors and Affiliations

  1. Xiangya Hospital, Central South University, Changsha, 410008, People’s Republic of China

    Shao Liu, Xin-Zhong Li, Li-Min Xu & Peng Lei

  2. Research Center for Modernization of Chinese Medicines, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, People’s Republic of China

    Shao Liu & Yi-Zeng Liang

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  1. Shao Liu
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  2. Xin-Zhong Li
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  3. Li-Min Xu
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  4. Peng Lei
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  5. Yi-Zeng Liang
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Correspondence to Xin-Zhong Li or Yi-Zeng Liang.

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Liu, S., Li, XZ., Xu, LM. et al. UPLC–MS–MS Analysis of Baicalin in the Cerebrospinal Fluid of Rabbits: Application to a Pharmacokinetic Study. Chroma 68, 463–466 (2008). https://doi.org/10.1365/s10337-008-0725-0

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  • DOI: https://doi.org/10.1365/s10337-008-0725-0

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