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Simultaneous Quantification of Seven Flavonoids in Flos Gossypii by LC

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Abstract

A simple, reproducible and accurate RP-LC method has been developed for simultaneous analysis of seven flavonoids (quercimeritrin, hyperoside, isoquercetrin, astragalin, quercetin, quercetin-3′-glucoside, and tiliroside) in Flos Gossypii (FG), a drug used in Xinjiang, China. The analytes were separated on a C18 column by elution with a gradient prepared from methanol, acetonitrile, and 0.2% aqueous phosphoric acid. The method was validated for linearity, accuracy, precision, sensitivity, and system suitability. It has been successfully used for evaluation of ten samples of different origin from Xinjiang, China. A similar pattern was observed for all the FG samples analyzed; quercimeritrin, isoquercetrin, and quercetin-3′-glucoside were the major compounds. The method is a simple, rapid, and reliable tool for quality control and may help reveal the correlation between the bioactivity of the FG and individual flavonoid content.

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Acknowledgments

The authors acknowledge financial support from National Key Technology R&D Program (2007BAI30B02) and High Technical Program of the Xinjiang, China (200711107).

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Authors and Affiliations

  1. Xinjiang Key Laboratory of Plant Resources and Natural Products Chemistry, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 830011, Urumqi, China

    Tao Wu, Rahima Abdulla, Yongxin Zhao, Yi Yang, Jun Chen & Haji Akber Aisa

  2. Graduate University of the Chinese Academy of Sciences, 100049, Beijing, China

    Tao Wu, Rahima Abdulla & Yi Yang

Authors
  1. Tao Wu
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  2. Rahima Abdulla
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  3. Yongxin Zhao
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  4. Yi Yang
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  5. Jun Chen
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  6. Haji Akber Aisa
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Correspondence to Haji Akber Aisa.

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Wu, T., Abdulla, R., Zhao, Y. et al. Simultaneous Quantification of Seven Flavonoids in Flos Gossypii by LC. Chroma 68, 467–470 (2008). https://doi.org/10.1365/s10337-008-0723-2

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  • DOI: https://doi.org/10.1365/s10337-008-0723-2

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