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Selective Densitometric Analysis of Cephalosporins Using Dragendorff’s Reagent

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Abstract

A simple, selective, precise, and stability-indicating thin-layer chromatographic method has been developed and validated for analysis of the cephalosporins cefpodoxime proxetil, ceftriaxone sodium, ceftazidime pentahydrate, cefotaxime sodium, cefoperazone sodium, cefazolin sodium, and cefixime in the bulk drug and in pharmaceutical formulations. TLC was performed on aluminium sheets precoated with silica gel G 60F254 as stationary phase. The mobile phases chosen for development gave compact spots for all the drugs (R F values 0.43–0.60). The separated compounds were visualized as orange spots by spraying with Dragendorff’s reagent. Linear regression analysis data for the calibration plots revealed good linear relationships between response and amounts of the drugs with correlation coefficients ranging from 0.9977 to 0.9998 and determination coefficients ranging from 0.9954 to 0.9996 over the concentration ranges 5–25 μg per spot for cefpodoxime proxetil, ceftriaxone sodium, and ceftazidime pentahydrate and 10–50 μg per spot for cefotaxime sodium, cefoperazone sodium, cefazolin sodium, and cefixime. The method was validated for precision, recovery, and robustness. Limits of detection and quantitation for the drugs ranged from 0.35 to 2.48 and from 1.07 to 7.50 μg per spot, respectively. The method was successfully applied to analysis of the drugs in their pharmaceutical dosage forms with good precision and accuracy. The method can also be used as a stability-indicating assay.

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Authors and Affiliations

  1. Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, 71526, Egypt

    Fardous A. Mohamed, Gamal A. Saleh, Salwa R. El-Shaboury & Azza H. Rageh

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  1. Fardous A. Mohamed
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  2. Gamal A. Saleh
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  3. Salwa R. El-Shaboury
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  4. Azza H. Rageh
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Correspondence to Azza H. Rageh.

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Mohamed, F.A., Saleh, G.A., El-Shaboury, S.R. et al. Selective Densitometric Analysis of Cephalosporins Using Dragendorff’s Reagent. Chroma 68, 365–374 (2008). https://doi.org/10.1365/s10337-008-0714-3

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