Skip to main content
Log in

Evaluation of Carbonyl Compounds in Natural Products by o-Dianisidine

  • Full Short Communication
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

A simple sensitive and rapid method is herein described for the qualitative and quantitative chemical assessment of the fractional composition of carbonyl functionalities in natural products. Several extracts, essential oils, fixed oils, oleoresins and pure natural isolates were directly analyzed by applying a simple colorimetric procedure, by LC or DE-TLC using o-dianisidine as a chromogenic reagent. The reagent is found to be very useful and could also be used in the microscopic examination of powdered herbal products.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Longo MA, Sanromán MA (2006) Food Technol Biotechnol 44(3):335–353

    CAS  Google Scholar 

  2. FAO (1995) Flavors and fragrances of plant origin, NON-WOOD FOREST PRODUCTS 1. FAO, Rome

  3. Facchetti R, Cadoppi A (2005) Characterization of essential oils by gas chromatography in one minute, Thermo Electron Corporation, Milan, Application Note 10024

  4. AL-Shaer M (2006) Reversed-phase HPLC determination of citral in locally grown lemon grass harvested at different season. Thesis, Master of Science in Chemistry, East Tennessee State University, USA

  5. Cserháti T, Forgács E, Deyl Z, Miksik I (2005) Biomed Chromatogr 19:183–190. Wiley InterScience http://www.interscience.wiley.com. doi:10.1002/bmc.486

  6. Schulz H, Baranska M, Belz HH, Rosch P, Strehle MA, Popp J (2004) Vib Spectrosc 35:81–86. doi:10.1016/j.vibspec.2003.12.014

    Article  CAS  Google Scholar 

  7. Dudareva N, Pichersky E, Gershenzon J (2004) Plant physiology. In: Biochemistry of plant volatiles, vol 135. pp 1893–1902

  8. B.P., 2007

  9. ISO 6885:2006 specifies a method for the determination of the anisidine value in animal and vegetable fats and oils. This is a measure of the amount of aldehydes present (principally alpha, beta-unsaturated aldehydes)

  10. Gupta MK (2003) Fundamental quality control in vegetable oil refining, Oil Mill Gazetteer vol 108. pp 6–9

  11. Hess AVI (2007) J Chem Educ 84:842

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Mohamed Abou-Shoer.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Abou-Shoer, M. Evaluation of Carbonyl Compounds in Natural Products by o-Dianisidine. Chroma 68, 447–451 (2008). https://doi.org/10.1365/s10337-008-0708-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-008-0708-1

Keywords

Navigation