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Efficient Protocol for Large-Scale Purification of Naringin with High Recovery from Fructus aurantii by Macroporous Resin Column Chromatography and HSCCC

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Abstract

Several kinds of resins were investigated in the first step and D101 macroporous resin was selected for cleaning-up naringin (NAR), a major flavonoid glycoside from Fructus aurantii. In the subsequent column chromatography, 10% aqueous ethanol was first used to elute the column to remove the undesired constituents and 70% aqueous ethanol was used to elute the target. The content of NAR was 57.1% with 95.7% recovery in this process. In the second step, the obtained crude sample was directly isolated by high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of ethyl acetate–n-butanol–water at a volume ratio of 2: 0.8: 3.2 (v/v/v), and 331 mg NAR with 98.3% purity was obtained from 600 mg crude extract in only one run. The recovery of the compound in this step was 95.0%. Thus, the total recovery of NAR was 90.9% after the two step purification. The established protocol for large-scale isolation and separation of NAR with high purity and recovery from F. aurantii was simple, efficient, and suitable for pharmace- utical and commercial use.

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Acknowledgments

This work was partly supported by Science and Technology Bureau of Dalian, China (excellent young scientist foundation no. 2006J23JH024).

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Authors and Affiliations

  1. College of Pharmacy, Dalian Medical University, No.9 West Part of Lvshun South Road, 116044, Dalian, China

    Xu Han, Lina Xu, Youwei Xu, Yan Qi, Yunpeng Diao, Jinyong Peng & Kexin Liu

  2. The First Affiliated Hospital of Dalian Medical University, 116011, Dalian, China

    Lingli Zheng

  3. Laboratory Animal Center, Dalian Medical University, 116044, Dalian, China

    Zewen Qiu

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  1. Xu Han
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  2. Lingli Zheng
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  3. Zewen Qiu
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  4. Lina Xu
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Correspondence to Jinyong Peng.

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Han, X., Zheng, L., Qiu, Z. et al. Efficient Protocol for Large-Scale Purification of Naringin with High Recovery from Fructus aurantii by Macroporous Resin Column Chromatography and HSCCC. Chroma 68, 319–326 (2008). https://doi.org/10.1365/s10337-008-0704-5

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  • DOI: https://doi.org/10.1365/s10337-008-0704-5

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