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Simultaneous Quantification of Six Sesquiterpene Lactones in Inula britannica L. by RP-LC

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Abstract

A reversed-phase high-performance liquid chromatographic method was developed for the first time to simultaneously determine six major sesquiterpene lactones in Inula britannica L, that is, britannilactone, 1-O-acetylbritannilactone, hydroxylantolactone, 8-epi-ivangustin, 6-isobutylbritannilactone and 1,6-O,O-diacetylbritannilactone. The chromatographic separation was achieved with a linear gradient of acetonitrile in water with a flow rate at 1.0 mL min−1 using an Inertsil ODS-3 column (250 × 4.6 mm, 5.0 μm). Detection was carried out using a photodiode array detector at 210 nm. The calibration curves for the determination of all analytes showed good linearity over the investigated ranges (R 2 > 0.999). Repeatability was evaluated by intra-day and inter-day assays and RSD values were less than 3.0%. The recoveries were between 93.9 and 104.6%. This developed method can be used for quality control purposes and allows for the differentiation between Inula britannica L., Inula hupehensis Ling., and Inula japonica Thunb.

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Acknowledgment

This work was supported by the Natural Science Fund of Hebei province (NO. 303470).

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Authors and Affiliations

  1. Department of Development of New Drugs, College of Pharmacy, Hebei Medical University, 361 East Zhongshan Road, 050017, Shijiazhuang, China

    Xiao-Wei Shi, Jin-Long Qi, Yi-Bing Wu, Yan Fu, Yun-Zhi Wang & Di-Qun Zhang

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  1. Xiao-Wei Shi
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  2. Jin-Long Qi
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  3. Yi-Bing Wu
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  4. Yan Fu
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  5. Yun-Zhi Wang
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Correspondence to Yun-Zhi Wang.

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Shi, XW., Qi, JL., Wu, YB. et al. Simultaneous Quantification of Six Sesquiterpene Lactones in Inula britannica L. by RP-LC. Chroma 68, 281–285 (2008). https://doi.org/10.1365/s10337-008-0698-z

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