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On-Line Screening and Identification of Radical Scavenging Compounds Extracted from Flos Lonicerae by LC-DAD–TOF-MS

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Abstract

Flos Lonicerae is commonly used in traditional Chinese medicine. The ethanolic extracts prepared from Flos Lonicerae were used for the rapid evaluation of free radical scavenging activity by using the extracts to clear on-line 2,2′-diphenyl-1-picrylhydrazyl (DPPH·) free radical with LC serving as the separating tool of bioactive compounds, two diode array detectors (DAD) and a time-of-flight mass spectrometer (TOF-MS) as qualitation tools. The LC-DPPH on-line method of Flos Lonicerae extracts revealed the presence of four radical scavenging compounds, which were simultaneously identified as chlorogenic acid, rutin, and two isomers of isochlorogenic acid by high-resolution TOF-MS. To the best of our knowledge, it is original to demonstrate the rapid and successful use of LC-DPPH-DAD–TOF-MS to directly and simultaneously screen and identify the anti-free radical compounds in the extracts of Flos Lonicerae without any purification. The present method provides a useful improvement on the reducing of workload

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Acknowledgements

The authors gratefully acknowledge the financial support of the National Natural Science Foundation of China (20675015), the foundation of National High Technology and Development of China (863 projects: 2006AA02Z4Z1 and 2007AA02Z4A4), the Fujian Provincial Education Department Foundation (JA07101 and 2005k049), and the foundation of the Key Laboratory of Chinese Pharmacies for Fujian University of Traditional Chinese Medicine of Fujian Higher Education (CKJ2007032ZYX200710)

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Authors and Affiliations

  1. Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, 350004, China

    Yan Chen, Shaoguang Li, Xinhua Lin, Hongbin Luo, Guangwen Li & Hong Yao

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  1. Yan Chen
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  2. Shaoguang Li
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  3. Xinhua Lin
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  4. Hongbin Luo
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  5. Guangwen Li
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Correspondence to Xinhua Lin or Hong Yao.

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Chen, Y., Li, S., Lin, X. et al. On-Line Screening and Identification of Radical Scavenging Compounds Extracted from Flos Lonicerae by LC-DAD–TOF-MS. Chroma 68, 327–332 (2008). https://doi.org/10.1365/s10337-008-0695-2

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