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Determination of Polychlorinated Biphenyl 126 in Liver and Adipose Tissues by GC-μECD with Liquid Extraction and SPE Clean-Up

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Abstract

Simple, rapid, accurate, and reproducible gas chromatographic methods were developed for the quantitative determination of PCB 126 in liver and adipose tissues. PCB 126 was extracted from the tissues by homogenizing the former with hexane and by alkaline hydrolysis of the latter with 2 N NaOH solution, respectively. Sample clean-up was conducted employing the Varian Bond Elut® PCB cartridge, using hexane as an eluant. The developed methods were validated for calibration curve linearity, accuracy, precision, and lower limit of quantitation. The applicability of these methods can be expanded to the analyses of other PCB congeners in similar biological tissues.

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References

  1. ATSDR (2000) Toxicological profile for polychlorinated biphenyls (PCBs). http://www.atsdr.cdc.gov/toxprofiles/tp17.pdf

  2. Li MH, Hansen LG (1997) Rev Toxicol 1:71–156

    CAS  Google Scholar 

  3. Safe SH (1994) Crit Rev Toxicol 24:87–149. doi:10.3109/10408449409049308

    Article  CAS  Google Scholar 

  4. Fisher JW, Campbell J, Muralidhara S, Bruckner JV, Ferguson D, Mumtaz M, Harmon B, Hedge JM, Crofton KM, Kim H, Almekinder TL (2006) Toxicol Sci 90:87–95. doi:10.1093/toxsci/kfj069

    Article  CAS  Google Scholar 

  5. Ferrario J, Byrne C, Dupuy A Jr (1997) Chemosphere 34:2451–2465. doi:10.1016/S0045-6535(97)00083-0

    Article  CAS  Google Scholar 

  6. Lyche JL, Skaare JU, Larsen HJS, Ropstad E (2004) Chemosphere 55:621–629. doi:10.1016/j.chemosphere.2003.11.024

    Article  CAS  Google Scholar 

  7. Mes J, Arnold DL, Bryce F (1995) Arch Environ Contam Toxicol 29:69–76. doi:10.1007/BF00213089

    Article  CAS  Google Scholar 

  8. Stefanelli P, Ausili A, Muccio AD, Fossi C, Muccio SD, Rossi S, Colasanti A (2004) Mar Pollut Bull 49:938–950. doi:10.1016/j.marpolbul.2004.06.019

    Article  CAS  Google Scholar 

  9. González-Barros C, Piñeiro MEA, Lozano JS, Yusty MAL (1996) Chromatographia 43:398–400. doi:10.1007/BF02271018

    Article  Google Scholar 

  10. Monosson E, Ashley JTF, McElroy AE, Woltering D, Elskus AA (2003) Chemosphere 52:777–787. doi:10.1016/S0045-6535(03)00228-5

    Article  CAS  Google Scholar 

  11. Bester K, Beernaert H, Bernreuther A, Harbeck S, Kinieczka P, Linsinger TPJ, Mertens I, Schimmel H, Sejerøe-Olsen B, Pauwels J, De Pooter G (2001) Anal Bioanal Chem 374:305–313. doi:10.1007/s00216-002-1417-5

    Article  Google Scholar 

  12. Covaci A, de Boer J, Ryan JJ, Voorspoels S, Schepens P (2002) Anal Chem 74:790–798. doi:10.1021/ac010784e

    Article  CAS  Google Scholar 

  13. Smrek AL, Needham LL (1982) Bull Environ Contam Toxicol 28:718–722. doi:10.1007/BF01605642

    Article  CAS  Google Scholar 

  14. Patterson DG, Todd GD, Turner WE, Maggio V, Alexander LR, Needham LL (1994) Environ Health Perspect Suppl 102:195–204

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Environmental Science, Kangwon National University, Chuncheon, Kangwon-do, 200-701, South Korea

    Hekap Kim

  2. Interdisciplinary Toxicology Program, University of Georgia, Athens, GA, 30602, USA

    Jeffrey W. Fisher

Authors
  1. Hekap Kim
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  2. Jeffrey W. Fisher
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Correspondence to Hekap Kim or Jeffrey W. Fisher.

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Kim, H., Fisher, J.W. Determination of Polychlorinated Biphenyl 126 in Liver and Adipose Tissues by GC-μECD with Liquid Extraction and SPE Clean-Up. Chroma 68, 307–309 (2008). https://doi.org/10.1365/s10337-008-0694-3

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  • DOI: https://doi.org/10.1365/s10337-008-0694-3

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