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Quantitative Analysis of Genistein in Human Plasma by Online Concentration CE with UV Detection

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Abstract

A rapid capillary electrophoresis method based on online acid barrage stacking has been successfully established for analysis of trace amounts of genistein in human plasma. Genistein was analyzed within 8 min, with 200 mmol L−1 citric acid (pH 1.7) as acid barrage, and injection times of 180 and 30 s for sample and acid, respectively. Good linearity was obtained in the range 0.05–5 mg L−1 and the limit of detection was 0.03 mg L−1. Compared with normal sample injection, this method resulted in more than a fiftyfold improvement in detection sensitivity. The technique has potential for use in studies of genistein metabolism and of exposure levels in humans.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 20235010, 20775088), the Foundation of State Key Laboratory of Electroanalytical Chemistry (No. 2006002), and the President Foundation of GUCAS.

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, The Graduate University of the Chinese Academy of Sciences, Beijing, 100049, China

    Hai-Yan Feng, Shu-Rong Hou, Na Zheng, Xiang-Jun Li, Zhong-Bo Hu & Zhuo-Bin Yuan

  2. School of Chemical Engineering, Shijiazhuang University, Shijiazhuang, 050035, China

    Hai-Yan Feng

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  1. Hai-Yan Feng
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  2. Shu-Rong Hou
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  3. Na Zheng
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  4. Xiang-Jun Li
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  5. Zhong-Bo Hu
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Correspondence to Xiang-Jun Li.

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Feng, HY., Hou, SR., Zheng, N. et al. Quantitative Analysis of Genistein in Human Plasma by Online Concentration CE with UV Detection. Chroma 68, 431–435 (2008). https://doi.org/10.1365/s10337-008-0658-7

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  • DOI: https://doi.org/10.1365/s10337-008-0658-7

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