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LC–MS–MS Analysis of Mirtazapine in Plasma, and Determination of Pharmacokinetic Data for Rats

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Abstract

A sensitive LC–MS–MS method with electrospray ionization has been developed for analysis of mirtazapine in rat plasma. After addition of diazepam as internal standard, liquid–liquid extraction was used to produce a protein-free extract. Chromatographic separation was achieved on a 150 × 4.6 mm, 5 μm particle, ODS column with 84:16 (v/v) methanol–water containing 0.1% ammonium acetate and 0.01% glacial acetic acid as mobile phase. LC–MS–MS was performed in selected-ion-monitoring (SIM) mode using target fragment ions m/z 195.09 for mirtazapine and m/z 192.80 for the IS. Calibration plots were linear over the range of 0.516–618.8 ng mL−1. The lower limit of quantification was 0.516 ng mL−1. Intra-day and inter-day precision were better than 12.6 and 8.8%, respectively. Mean recovery of mirtazapine from plasma was in the range 87.41–90.06%; average recovery was 88.40% (RSD 3.95%). Significant gender differences between mirtazapine pharmacokinetic data were observed in this study.

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Authors and Affiliations

  1. Nanjing Medical University Affiliated Nanjing Brain Hospital, 210029, Nanjing, China

    Xiao Hong, Yao Yao & Shen Hong

  2. Department of Pharmaceutical Analysis, China Pharmaceutical University, 210009, Nanjing, China

    Chen Lei

Authors
  1. Xiao Hong
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  2. Yao Yao
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  3. Shen Hong
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  4. Chen Lei
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Correspondence to Xiao Hong.

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Hong, X., Yao, Y., Hong, S. et al. LC–MS–MS Analysis of Mirtazapine in Plasma, and Determination of Pharmacokinetic Data for Rats. Chroma 68, 65–70 (2008). https://doi.org/10.1365/s10337-008-0656-9

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  • DOI: https://doi.org/10.1365/s10337-008-0656-9

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