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Rapid TLC Method for Estimation of Mevalonic Acid in the Leaves of Medicinal Plants

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Abstract

A simple, rapid, specific and sensitive thin layer chromatographic (TLC) method has been developed for the quantitative estimation of mevalonic acid (MVA) in leaves of medicinal plants; Artemisia annua, Psorelia corylifolia, Vinca rosea, Withania somnifera and Barleria proinites. The assay procedure involved conversion of MVA to its lactone, mevalonolactone (MVAL). Mevalonic acid was extracted from the leaf tissues of plants. Separation of MVAL was carried out on silica gel 60 F254 TLC plates using benzene:acetone (3:2) as the mobile phase. The densitometric determination of MVAL was performed at 600 nm after derivatization with anisaldehyde reagent in absorption–reflectance mode. The method was validated over the linearity range of 100–500 ng spot−1 and correlation coefficient for the calibration curve was >0.99. The average recovery of MVAL, used as internal standard, was higher than 98%. The lower limit of detection was found to be 50 ng spot−1.

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Acknowledgments

This work was supported by a research grant from the Department of Biotechnology, Ministry of Science and Technology, Govt. of India. One of the authors, Mr. Mauji Ram, thanks DBT for providing the financial assistance in the form of a Junior Research Fellowship.

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Authors and Affiliations

  1. Centre for Transgenic Plant Development, Department of Biotechnology, Jamia Hamdard, New Delhi, 110062, India

    Mauji Ram, Salim Khan, Mather A. Khan, Usha Kiran & Malik Z. Abdin

  2. Department of Biochemistry, Jamia Hamdard, New Delhi, 110062, India

    Saleem Javed

  3. Department of Botany, Jamia Hamdard, New Delhi, 110062, India

    Prabhakar Jha

Authors
  1. Mauji Ram
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  2. Salim Khan
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  3. Prabhakar Jha
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  4. Mather A. Khan
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  5. Usha Kiran
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  6. Saleem Javed
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  7. Malik Z. Abdin
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Corresponding author

Correspondence to Malik Z. Abdin.

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Ram, M., Khan, S., Jha, P. et al. Rapid TLC Method for Estimation of Mevalonic Acid in the Leaves of Medicinal Plants. Chroma 68, 129–133 (2008). https://doi.org/10.1365/s10337-008-0651-1

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  • DOI: https://doi.org/10.1365/s10337-008-0651-1

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