Abstract
A simple liquid chromatographic method was developed for the separation and simultaneous determination of cobalt and nickel as chelates with 1-(2-pyridylazo)-2-naphthol (PAN). The method, using a switching column technique for the on-line purification and separation, enables to reach the sub-microgram per litre concentration level excluding off-line sample treatment with the exception of the derivatization reaction. Two small-sized columns packed with CN- and C4-bonded stationary phases were selected and used considering their complementary behaviour with respect to chelated Co and Ni ions. The analysis was performed within 10 min using an optimised eluent (water–acetonitrile–methanol–tetrahydrofuran, 40:45:10:5, v/v/v/v) containing Tween 40 (10−3 M) and acetate buffer (5 × 10−3 M, pH 4.8). Detection was performed by UV-vis spectrophotometry (λ = 565 nm) permitting to reach quantification limits of 0.9 and 0.5 μg L−1 for Co and Ni, respectively.
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References
Friberg L, Nordberg G, Vouk V (1986) In: Handbook on the toxicology of metals V (eds) II: specific metals, 2nd edn. Elsevier Science Publishers, Amsterdam
Achterberg EP (2000) Int J Environ Pollut 13:249–261
Ostapczuk P, Stoeppler M, Duerbeck HW (1988) Fresenius Z Anal Chem 332:662–665
Herzog G, Arrigan DWM (2005) Trends Anal Chem 24:208–217
Isaac RA, Johnson WC (1998) In: Kalra YP (ed) Handbook of reference methods for plant analysis. CRC, Boca Raton, pp 165–170
Wang J, Hansen EH, Miro M (2003) Anal Chim Acta 499:139–147
Moldovan M, Krupp EM, Holliday AE, Donard OFX (2004) J Anal Atom Spectrom 19:815–822
Wang J, Hansen EH (2003) Trends Anal Chem 22:836–846
Kuban P, Guchardi R, Hauser PC (2005) Trends Anal Chem 24:192–198
Malik AK, Kaur V, Verma N (2006) Talanta 68:842–849
Szpunar J (2000) Trends Anal Chem 19:127–137
Ferrarello CN, Fernandez de la Campa MR, Sanz-Medel A (2002) Anal Bioanal Chem 373:412–421
O’Laughlin JW (1984) J Liq Chromatogr 7:127–204
Timerbaev AR, Petrukhin OM, Alimarin IP, Bol’shova TA (1991) Talanta 38:467–476
Jones P, Nesterenko PN (1997) J Chromatogr A 789:413–435
Schwedt G, Budde R (1982) Chromatographia 15:527–529
Inoue H, Ito K (1994) Michrochem J 49:249–255
Yasui T, Yuchi A, Yamada H, Wada H (1994) J Chromatogr A 659:359–365
Ryan E, Meaney M (1992) Analyst 117:1435–1439
Uehara N, Fukuda K, Shijo Y (1998) Anal Sci 14:343–347
Afkhami A, Bahram M (2006) Microchim Acta 155:403–408
Nabi S, Alim A, Islam A, Amjad M (2005) J Sep Sci 28:2463–2467
Galik A (1971) Anal Chim Acta 57:399–414
Pramauro E, Prevot A.B, Zelano V, Hinze WL, Viscardi G, Savarino P (1994) Talanta 41:1261–1267
Ramsteiner KA (1988) J Chromatogr 456:3–20
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This work has been partly supported by the FNS (France) under the program ACI-ECD (project “Diagnos-Métaux-Urine”).
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Tzanis, L., Simon, P. & Walcarius, A. Simultaneous Determination of Nickel and Cobalt as Chelates with 1-(2-Pyridylazo)-2-naphthol Using an LC Switching Column Method. Chroma 67, 857–861 (2008). https://doi.org/10.1365/s10337-008-0632-4
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DOI: https://doi.org/10.1365/s10337-008-0632-4