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Simultaneous Determination of Four Major Iridoid Glycosides in Scrophularia ningpoensis by CE

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Abstract

A simple, accurate and reproducible capillary electrophoresis method with UV detection has been developed for the simultaneous determination of four iridoid glycosides, 6-O-methyl-catalpol, aucubin, harpagide, and harpagoside, in Scrophularia ningpoensis (Xuan-shen). The running buffer was 100 mM borate (pH 9.3) containing 20% methanol. Applied voltage was 20 kV and temperature was 25 °C. Diphylloside A was used as an internal standard (IS) and detection was at 200 nm. The effects on separation of buffer pH, buffer concentration, and organic modifiers were investigated. The extracts of S. ningpoensis were well separated within 45 min.

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Acknowledgements

The authors gratefully acknowledge the Agriculture Science Institution of Zhenping Ankang for the collection of plant material and Chen yan for help with the experimental procedure.

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Authors and Affiliations

  1. Provincial Key Laboratory of Biomedicine, Northwest University, Xi’an, People’s Republic of China

    Jing Li, Xiaoyan Huang, Daowan Lai & Wenji Sun

  2. Drug inspection institute of Shaanxi, Shaanxi, People’s Republic of China

    Ji Li

Authors
  1. Jing Li
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  2. Xiaoyan Huang
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  3. Daowan Lai
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  4. Ji Li
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  5. Wenji Sun
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Correspondence to Wenji Sun.

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Li, J., Huang, X., Lai, D. et al. Simultaneous Determination of Four Major Iridoid Glycosides in Scrophularia ningpoensis by CE. Chroma 67, 989–993 (2008). https://doi.org/10.1365/s10337-008-0631-5

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  • DOI: https://doi.org/10.1365/s10337-008-0631-5

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