Skip to main content

Investigation of Euphrasia rostkoviana Hayne Using GC–MS and LC–MS

Chromatographia volume 68, pages 119–124 (2008)Cite this article

Abstract

Gas and liquid chromatography coupled with mass spectrometry was applied to solve difficulties and reinvestigate the serious matrix problems affecting analysis of the active compounds in Euphrasia rostkoviana Hayne. The main groups of compounds were obtained by extracting the herb stepwise with n-hexane, chloroform, ethyl acetate and methanol. Polyamide column chromatography facilitated further separation. Phenolic/flavonoid- and terpenoid-type molecules were studied by GC–MS, HPLC and LC–MS–MS. The β-sitosterol content of the herb was determined by gas chromatography with flame ionisation detection (GC-FID). Caffeic acid, chlorogenic acid, coumaric acid and flavonoid glycosides of apigenin, luteolin, rhamnetine (hexoside), kaempferol (both hexoside and rutinoside) and quercetin (rutinoside) were identified in the fractions of the methanolic extract.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

39,95 €

Price includes VAT (Finland)

Fig. 1
Fig. 2
Fig. 3

References

  1. Grainger Bisset N (1994) Herbal drugs and phytopharmaceuticals. CRC Press, medpharm Scientific Publishers, Stuttgart

    Google Scholar 

  2. Duke JA, (1985) Eyebright. Handbook of medicinal herbs. CRC Press, Boca Raton

    Google Scholar 

  3. Stoss M, Michels C, Peter E, Beutke R, Gorter RW (2000) J Alternat Complement Med 6(6):499–508

    Article  CAS  Google Scholar 

  4. Blaschek W, Hänsel R, Keller K, Reichling J, Rimpler H, Schneider G (1998) Hagers Handbuch der Pharmazeutischen Praxis. Folgewerk, Springer, Berlin

    Google Scholar 

  5. Matlawska I, Sikorska M, Kowalewski Z (1985) Herba Pol 31:119–123

    Google Scholar 

  6. Matlawska I, Sikorska M, Kowalewski Z (1988) Herba Pol 34:97–102

    CAS  Google Scholar 

  7. Petrichenko VM, Sukhinina TV, Babiyan LK, Shramm NI (2006) Pharm Chem J 40:22–26

    Article  CAS  Google Scholar 

  8. Luczak S, Swiatek L (1990) Plantes Med Phytother 24:66–70

    CAS  Google Scholar 

  9. Harkiss KJ, Timmins P (1973) Planta Med 23:342–347

    Article  CAS  Google Scholar 

  10. Sticher O, Salama O (1981) Planta Med 42:122–123

    Article  CAS  Google Scholar 

  11. Sticher O, Salama O (1981) Planta Med 42:123–124

    Article  Google Scholar 

  12. Salama O, Sticher O (1983) Planta Med 47:90–94

    Article  CAS  Google Scholar 

  13. Vukics V, Hevesi TB, Fukász Á, Kéry Á (2006) Chromatographia 63:125–129

    Article  CAS  Google Scholar 

  14. Kazuno S, Yanagida M, Shindo N, Murayama K (2005) Anal Biochem 347:182–192

    Article  CAS  Google Scholar 

  15. Hvattum H, Ekeberg D (2003) J Mass Spectrom 38:43–49

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacognosy, Semmelweis University, Üllői u. 26, Budapest, 1085, Hungary

    Balazs Blazics, Szabolcs Szarka & Agnes Kery

  2. Department of Pharmaceutics, Semmelweis University, Hőgyes E. u. 7, Budapest, 1092, Hungary

    Krisztina Ludanyi

Authors
  1. Balazs Blazics
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Krisztina Ludanyi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Szabolcs Szarka
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Agnes Kery
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Balazs Blazics.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Blazics, B., Ludanyi, K., Szarka, S. et al. Investigation of Euphrasia rostkoviana Hayne Using GC–MS and LC–MS. Chroma 68 (Suppl 1), 119–124 (2008). https://doi.org/10.1365/s10337-008-0630-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-008-0630-6

Keywords

  • Column liquid chromatography–mass spectrometry
  • Gas chromatography–mass spectrometry
  • Euphrasia rostkoviana
  • Flavonoids