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Simultaneous LC–UV Determination of 5-Methyl-1-(3-fluorophenyl)-2-(1H)- pyridone and Its Two Metabolites in Rat Plasma

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Abstract

A simple, rapid, and reproducible isocratic reverse-phase HPLC method was developed to simultaneously determine AKF-PD and its two oxidized metabolites in rat plasma. 5-Carboxyl-1-phenyl-2-(1H)-pyridone and phenacetin were used as internal standards to ensure the precision and accuracy of the method. The analytes were separated on a C18 reversed-phase column with methanol—phosphate buffer (20 mM, pH 2.5) as mobile phase. The limits of detection for AKF-PD and its two oxidized metabolites was 0.1 μg mL−1. The method is applicable for the pharmacokinetic studies of AKF-PD and its metabolites in rats.

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References

  1. Al-Bayati MA, Xie Y, Mohr FC, Margolin SB (2002) Biochem Pharmacol 64:517–525

    Article  CAS  Google Scholar 

  2. Gahl WA, Brantly M, Troendle J, Avila NA (2002) Mol Gen Metab 76:234–242

    Article  CAS  Google Scholar 

  3. Iyer SN, Wild JS, Schiedt MJ, Hyde DM, Margolin SB, Giri SN (1995) J Lab Clin Med 125:779–785

    CAS  Google Scholar 

  4. Raghu G, Johnson WC, Lockhart D, Mageto Y (1999) Am J Respir Crit Care Med 159:1061–1069

    CAS  Google Scholar 

  5. Giri SN, Wang Q, Xie Y, Lango J, Morin D, Margolin SB, Buckpitt AR (2002) Biopharm Drug Dispos 23:203–211

    Article  CAS  Google Scholar 

  6. Cao W, He L, Liu W, Huang Z, Cheng Z (2006) J Chromatogr B 833:257–259

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to thank Ministry of Education of the People’s Republic of China and the Science and Technology Bureau of Hunan Province for funding.

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Authors and Affiliations

  1. School of Pharmaceutical Sciences, Central South University, 410013, Changsha, People’s Republic of China

    Xiaoai He, Gaoyun Hu, Zhi Liu, Wei Cao, Xiaolei Hu, Lijian Tao & Zeneng Cheng

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  1. Xiaoai He
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  2. Gaoyun Hu
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  3. Zhi Liu
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  4. Wei Cao
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  5. Xiaolei Hu
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  6. Lijian Tao
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  7. Zeneng Cheng
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Corresponding author

Correspondence to Zeneng Cheng.

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He, X., Hu, G., Liu, Z. et al. Simultaneous LC–UV Determination of 5-Methyl-1-(3-fluorophenyl)-2-(1H)- pyridone and Its Two Metabolites in Rat Plasma. Chroma 67, 947–950 (2008). https://doi.org/10.1365/s10337-008-0627-1

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  • DOI: https://doi.org/10.1365/s10337-008-0627-1

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