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Determination of Piribedil in Human Serum, Urine and Pharmaceutical Dosage Form by LC-DAD

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Abstract

A rapid, selective and sensitive reversed-phase liquid chromatographic (LC) method was developed for the determination of piribedil in human serum, urine and pharmaceutical dosage form. LC analysis was carried out using reversed-phase isocratic elution with a C18 column and a mobile phase of 0.01 M phosphate buffer-acetonitrile (50:50, v/v). The chromatograms showed good resolution and sensitivity with no interference of human serum and urine. Piribedil concentrations were determined using diode array detection at 240 nm. Sildenafil citrate was used as internal standard. The limit of quantification (LOQ) and limit of detection (LOD) concentrations were 107.2 and 321.6 pg mL−1, 96.6 and 290.4 pg mL−1, 161.7 and 53.9 pg mL−1 for urine, serum and pharmaceutical dosage forms, respectively. The method was validated for its linearity, precision and accuracy and applied to the tablets, urine and human serum. In addition, the results were compared to those obtained from UV-spectrophotometry.

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Authors and Affiliations

  1. Department of  Analytical Chemistry, Faculty of  Pharmacy, Anadolu University, 26470 , Eskisehir, Turkey

    Göksel Altiokka & Nafiz Ö. Can

  2. Department of  Pharmaceutical and Medicinal Chemistry, National Research Centre, Dokki, Cairo, 12311, Egypt

    Hassan Y. Aboul-Enein

Authors
  1. Göksel Altiokka
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  2. Nafiz Ö. Can
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  3. Hassan Y. Aboul-Enein
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Corresponding author

Correspondence to Hassan Y. Aboul-Enein.

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Altiokka, G., Can, N.Ö. & Aboul-Enein, H.Y. Determination of Piribedil in Human Serum, Urine and Pharmaceutical Dosage Form by LC-DAD. Chroma 67, 905–910 (2008). https://doi.org/10.1365/s10337-008-0626-2

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  • DOI: https://doi.org/10.1365/s10337-008-0626-2

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