Skip to main content
SpringerLink
Log in

LC Separation and Determination of Five Diester-Diterpenoid Alkaloids in the Unprocessed and Processed Aconite Roots

  • Limited Short Communication
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

A reliable liquid chromatographic method with photodiode array detector (DAD) was developed and validated for simultaneous separation and determination of five diester-diterpenoid alkaloids in the aconite roots. The separation was successfully performed on a Zorbax Extend-C18 column with a mobile phase gradient prepared from methanol and ammonia solution at a flow rate of 1.0 mL min−1. Good linearity (r > 0.999) was observed over the concentration ranges investigated, and intra-day and inter-day precision were high. The mean recoveries of five components ranged from 90.45 to 102.63% and relative standard deviations were always <5%. The validated method was successfully used for simultaneous determination of the five diester-diterpenoid alkaloids of unprocessed and processed aconite roots. The quantitative method provided a scientific basis for safety assurance and clinical application of aconite roots.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Chemical Industry Publishing House (2005) Pharmacopoeia of the People’s Republic of China. Beijing

  2. Wang Y, Liu Z, Song F, Liu S (2002) Rapid Commun Mass Spectrom 16:2075–2082

    Article  CAS  Google Scholar 

  3. Wang Y, Shi L, Song F, Liu Z, Liu S (2003) Rapid Commun Mass Spectrom 17:279–284

    Article  CAS  Google Scholar 

  4. Shim SH, Kim JS, Kang SS, Son KH, Bae K (2003) Arch Pharm Res 26:709–715

    Article  CAS  Google Scholar 

  5. Fu M, Wu M, Qiao Y, Wang Z (2006) Pharmazie 61:735–741

    CAS  Google Scholar 

  6. Lin CC, Chan TY, Deng JF (2004) Ann Emerg Med 43:574–579

    Article  Google Scholar 

  7. Moog FP, Karenberg A (2002) Adverse Drug React Toxicol Rev 21:151–156

    CAS  Google Scholar 

  8. Ameri A (1998) Prog Neurobiol 56:211–235

    Article  CAS  Google Scholar 

  9. Hikino H, Konno C, Takata H, Yamada Y, Yamada C, Ohizumi Y, Sugio K, Fujimura H (1980) J Pharmacobiodyn 3:514–525

    CAS  Google Scholar 

  10. Gutser UT, Friese J, Heubach JF, Matthiesen T, Selve N, Wilffert B, Gleitz J (1998) Naunyn Schmiedebergs Arch Pharmacol 357:39–48

    Article  CAS  Google Scholar 

  11. Tong YY, Wang K (1990) Yao Xue Xue Bao 25:387–389

    Google Scholar 

  12. Zhao S, Pan Z, Chen X, Hu Z (2004) Biomed Chromatogr 18:381–387

    Article  CAS  Google Scholar 

  13. Feng HT, Li SF (2002) J Chromatogr A 973:243–247

    Article  CAS  Google Scholar 

  14. Xie Y, Jiang ZH, Zhou H, Xu HX, Liu L (2005) J Chromatogr A 1093:195–203

    Article  CAS  Google Scholar 

  15. Yin J, Guo W, Du Y, Wang E (2006) Electrophoresis 27:4836–4841

    Article  CAS  Google Scholar 

  16. Xie Y, Jiang ZH, Zhou H, Xu HX, Liu L (2005) J Chromatogr A 1093:195–203

    Article  CAS  Google Scholar 

  17. Wang Z, Wen J, Xing J, He Y (2006) J Pharm Biomed Anal 40:1031–1034

    Article  CAS  Google Scholar 

  18. Jiang ZH, Xie Y, Zhou H, Wang JR, Liu ZQ, Wong YF, Cai X, Xu HX, Liu L (2005) Phytochem Anal 16:415–421

    Article  CAS  Google Scholar 

  19. Liu XX, Chao RB (2006) Yao Xue Xue Bao 41:365–369

    CAS  Google Scholar 

  20. Wu W, Liang Z, Zhao Z, Cai Z (2007) J Mass Spectrom 42:58–69

    Article  CAS  Google Scholar 

  21. Zhao C, Li M, Luo Y, Wu W (2006) Carbohydr Res 341:485–491

    Article  CAS  Google Scholar 

  22. Tang WT, Wong SK, Law TY, Pang KC, Sin D, Tam YK (2006) J AOAC Int 89:1496–1514

    CAS  Google Scholar 

  23. Li R, Wu Z, Zhang F, Ding L (2006) Rapid Commun Mass Spectrom 20:157–170

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work has been carried out with the financial support from the Scientific Plan Program of the National Science fund of China (2006BAI08B03-07).

Author information

Authors and Affiliations

  1. School of Pharmacy, Second Military Medical University, Shanghai, 200433, China

    Min Liu, Baiyun Zhao, Lingling Dong, Zhenyu Zhu & Yifeng Chai

  2. Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Shanghai, 200438, China

    Hai Zhang & Liang Zhao

Authors
  1. Min Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hai Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Liang Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Baiyun Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Lingling Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Zhenyu Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yifeng Chai
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yifeng Chai.

Additional information

The authors Min Liu and Hai Zhang equally contributed to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, M., Zhang, H., Zhao, L. et al. LC Separation and Determination of Five Diester-Diterpenoid Alkaloids in the Unprocessed and Processed Aconite Roots. Chroma 67, 1003–1006 (2008). https://doi.org/10.1365/s10337-008-0624-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-008-0624-4

Keywords

Search

Navigation