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New Mab CB.Hep-1 Purification Process Eliminates the Need for Pre-Chromatographic Purification. Stability Demonstrated Over 100 Purification Cycles

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Abstract

This investigation sought to discover whether purification of monoclonal antibody CB.Hep-1 in ascitic fluid is possible by protein A–Sepharose affinity chromatography, for 100 runs, without pre-purification steps. Results showed that direct application of ascitic fluid to protein A–Sepharose increased monoclonal antibody recovery by 27% compared with the traditional process (control) after 100 runs. The purity of the monoclonal antibody was >95% and the cost of the purification was 15% less than that of the control process. In conclusion, monoclonal antibody CB.Hep-1 in ascitic fluid can be purified by chromatography on protein A–Sepharose, for 100 purification cycles, without the need for pre-purification steps.

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Acknowledgments

The authors would like to thank to the Monoclonal Antibody Department of the Center for Genetic Engineering and Biotechnology for their contribution to this comparison study. The authors also gratefully acknowledge Mena Cayetana Ramos from ‘Salvador Allende’ hospital for her effort to make possible the publication of this manuscript.

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Authors and Affiliations

  1. Monoclonal Antibody Production Department, Center for Genetic Engineering and Biotechnology, Ave 31 be/158 and 190, P.O. Box 6162, 10600, Havana, Cuba

    Rodolfo Valdés, Tatiana Álvarez, Andrés Tamayo, José Montero, William Ferro, Yenisley Medina, Yarysel Guevara, Sigifredo Padilla, Dobián Cecilia, Lamay Dorta & Marcos González

  2. Inorganic Chemistry Department, Center for Engineering and Chemical Research, F Street, #115 be/Ave 5th and Calzada, 10400, Havana, Cuba

    Eutimio G. Fernandez

  3. Tobacco Research Institute, Km 8.½ Tumbadero Road, San Antonio de los Baños, Havana, Cuba

    Déborah Geada

  4. Process Control Department, Center for Genetic Engineering and Biotechnology, Ave 31 be/158 and 190, P.O. Box 6162, 10600, Havana, Cuba

    Raudel Sosa & Tatiana González

Authors
  1. Rodolfo Valdés
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  2. Tatiana Álvarez
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  3. Andrés Tamayo
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  4. Eutimio G. Fernandez
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  5. José Montero
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  6. Déborah Geada
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  7. William Ferro
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  8. Yenisley Medina
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  9. Yarysel Guevara
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  10. Sigifredo Padilla
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  11. Dobián Cecilia
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  12. Lamay Dorta
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  13. Marcos González
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  14. Raudel Sosa
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  15. Tatiana González
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Corresponding author

Correspondence to Eutimio G. Fernandez.

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Valdés, R., Álvarez, T., Tamayo, A. et al. New Mab CB.Hep-1 Purification Process Eliminates the Need for Pre-Chromatographic Purification. Stability Demonstrated Over 100 Purification Cycles. Chroma 67, 923–927 (2008). https://doi.org/10.1365/s10337-008-0607-5

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  • DOI: https://doi.org/10.1365/s10337-008-0607-5

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