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Evaluation of a TLC Densitometric Method for Analysis of Azole Antifungal Agents

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Abstract

A new method for identification and quantitative determination of the azole antifungal agents; ketoconazole, bifonazole, fluconazole and itraconazole is described using TLC with densitometric detection. Validation of the method was carried out to confirm its precision (%RSD ranged between 1.31 and 3.45), recovery (99.7–102.4%) and linearity (r  = 0.99287–0.99722) within the concentration range under investigation. The experimental conditions obtained enable the method to be used for both qualitative and quantitative pharmaceutical analysis.

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Authors and Affiliations

  1. Department of Inorganic and Analytical Chemistry, Collegium Medicum, Jagiellonian University, 9 Medyczna St., 30-688, Kraków, Poland

    Radosław J. Ekiert, Jan Krzek & Włodzimierz Rzeszutko

Authors
  1. Radosław J. Ekiert
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  2. Jan Krzek
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  3. Włodzimierz Rzeszutko
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Correspondence to Jan Krzek.

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Ekiert, R.J., Krzek, J. & Rzeszutko, W. Evaluation of a TLC Densitometric Method for Analysis of Azole Antifungal Agents. Chroma 67, 995–998 (2008). https://doi.org/10.1365/s10337-008-0604-8

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  • DOI: https://doi.org/10.1365/s10337-008-0604-8

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