, Volume 67, Issue 9–10, pp 783–788 | Cite as

Comparison of LC and UPLC Coupled to MS–MS for the Determination of Sulfonamides in Egg and Honey

Full Short Communication


Determination of ten sulfonamides (SAs) in egg and honey has been compared using column liquid chromatography (LC) and ultra-performance liquid chromatography (UPLC) coupled to tandem mass spectrometry (MS–MS). A liquid–liquid extraction with acetonitrile followed by solid-phase extraction on a Strata-X cartridge was developed for sample preparation. The analytical performance of both methods was compared applying the alternative matrix-comprehensive in-house validation approach using specially designed software InterVal™. Using UPLC the separation time was shortened about 30% reducing the run time by 8 min and a better resolution was achieved compared to LC. Due to higher peak efficiency achieved with UPLC, the decision limit values obtained by both techniques were almost equal (6.61–9.43 μg kg−1 and 7.25–11.9 μg kg−1 for UPLC and LC, respectively), despite the fact that in UPLC twice lower sample volumes were injected. Satisfactory and comparable recoveries (80–110%) were obtained by UPLC and LC for all the SAs, except for sulfacetamide by LC and sulfabenzamide by both methods. For a majority of the spiked compounds, UPLC gave significantly better precision.


Column liquid chromatography Ultra-performance LC Tandem mass spectrometry Sulfonamides Egg and honey 


  1. 1.
    Crosby NT (1991) In: Determination of veterinary residues in food. Ellis Horwood, ChichesterGoogle Scholar
  2. 2.
    Botsoglou NA, Fletouris DJ (2001) In: Drug residues in foods: pharmacology, food safety and analysis. Marcel Dekker, New YorkGoogle Scholar
  3. 3.
    Doerge DR, Decker CJ (1994) Chem Res Toxicol 7:164–169CrossRefGoogle Scholar
  4. 4.
    Commission of the European Communities, Council Regulation EEC 2377/90, Off. J. L224 (1990) 1Google Scholar
  5. 5.
    Sheth AB, Sporns P (1990) J Assoc Off Anal Chem 73:871–874Google Scholar
  6. 6.
    Neidert E, Baraniak Z, Sauve A (1986) J Assoc Off Anal Chem 69:641–645Google Scholar
  7. 7.
    Takatsuki K, Kikuchi T (1990) J Assoc Off Anal Chem 73:886–892Google Scholar
  8. 8.
    Kishida K, Furusawa N (2001) J Chromatogr A 973:49–55CrossRefGoogle Scholar
  9. 9.
    Malintan NT, Mohd MA (2006) J Chromatogr A 1127:154–160CrossRefGoogle Scholar
  10. 10.
    Abian J, Churchwell MI, Korfmacher WA (1993) J Chromatogr 629:267–276CrossRefGoogle Scholar
  11. 11.
    Haller MY, Müller SR, McArdell CS, Alder AC, Suter MJF (2002) J Chromatogr A 952:111–120CrossRefGoogle Scholar
  12. 12.
    Bogialli S, Curini R, Di Corcia A, Nazzari M, Polci ML (2003) J Agric Food Chem 51:4225–4232CrossRefGoogle Scholar
  13. 13.
    Doerge DR, Bajic S, Lowes S (1993) Rapid Commun Mass Spectrom 7:1126–1130CrossRefGoogle Scholar
  14. 14.
    Van Eeckhout N, Perez JC, Peteghem C (2000) Rapid Commun Mass Spectrom 14:2331–2338CrossRefGoogle Scholar
  15. 15.
    Shao B, Dong D, Wu Y, Hu J, Meng J, Tu X, Hu S (2005) Anal Chim Acta 546:174–181CrossRefGoogle Scholar
  16. 16.
    Potter RA, Burns BG, Van de Riet JM, North DH, Darvesh R (2007) J AOAC Int 90:343–348Google Scholar
  17. 17.
    Heller DN, Ngoh MA, Donoghue D, Podhorniak L, Righter H, Thomas MH (2002) J Chromatogr B 774:39–52CrossRefGoogle Scholar
  18. 18.
    Verzegnassi L, Savoy-Perroud MC, Stadler RH (2002) J Chromatogr A 977:77–87CrossRefGoogle Scholar
  19. 19.
    Thompson TS, Noot DK (20005) Anal Chim Acta 551:168–176CrossRefGoogle Scholar
  20. 20.
    Sherma J, Larkin JD, Larkin FH (2005) J AOAC Int 88:63A–67AGoogle Scholar
  21. 21.
    Swartz M (2005) J Liq Chromatogr Relat Technol 28:1253–1263CrossRefGoogle Scholar
  22. 22.
    Uhlig S, Gowik P, Radeck W (2003) Anal Chim Acta 483:351–362CrossRefGoogle Scholar
  23. 23.
    Bock C, Stachel C, Gowik P (2007) Anal Chim Acta 586:348–358CrossRefGoogle Scholar
  24. 24.
    Maudens KE, Zhang GF, Lambert WE (2004) J Chromatogr A 1047:85–92CrossRefGoogle Scholar
  25. 25.
    Ito Y, Oka H, Ikai Y, Matsumoto H, Miyazaki Y, Nagase H (2000) J Chromatogr A 898:95–102CrossRefGoogle Scholar
  26. 26.
    Krivohlavek A, Šmit Z, Baštinac M, Žuntar I, Plavšic-Plavšic FP (2005) J Sep Sci 28:1434–1439CrossRefGoogle Scholar
  27. 27.
    Hela W, Brandtner M, Widek R, Schuh R (2003) Food Chem 83:601–608CrossRefGoogle Scholar
  28. 28.
    Zotou A, Vasiliadou C (2006) Chromatographia 64:307–311CrossRefGoogle Scholar
  29. 29.
    Fuh MRS, Chu SY (2003) Anal Chim Acta 499:215–221CrossRefGoogle Scholar
  30. 30.
    Tarbin JA, Clarke P, Shearer G (1999) J Chromatogr B 729:127–138CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2008

Authors and Affiliations

  1. 1.Department of Analytical and Environmental ChemistryVilnius UniversityVilniusLithuania

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