, Volume 67, Issue 7–8, pp 621–625 | Cite as

LC–ESI–MS Determination of Lovastatin in Human Plasma

  • Lin Li
  • Jin Sun
  • Yaxin Sun
  • Zhonggui HeEmail author
Full Short Communication


A convenient, selective and sensitive liquid chromatographic-electrospary ionization mass spectrometry (LC–ESI–MS) method was developed and validated to determine lovastatin in human plasma. The analyte was extracted from human plasma samples by typical liquid–liquid extraction, separated on a C18 column by using the mobile phase consisting of water–methanol (13:87, v/v). Simvastatin was used as the internal standard (IS). The method was linear within the range of 0.1–20 ng mL−1. The lower limit of quantification (LLOQ) was 0.1 ng mL−1. The intra- and inter-run precision, calculated from quality control (QC) samples was less than 10.2%. The accuracy as determined from QC samples was in the range of 99.3–102.9% for the analyte. The mean recoveries for lovastatin and IS were 84.8 and 88.0%, respectively. The method was successfully applied for evaluation of the pharmacokinetic of lovastatin in healthy volunteers.


Column liquid chromatography LC–ESI–MS Pharmacokinetics study Human plasma Lovastatin 


  1. 1.
    Iulian MH, Rennie CH (1998) Drugs 36:429–431Google Scholar
  2. 2.
    Ramzi S (1988) Am J Med Sci 296:426–428CrossRefGoogle Scholar
  3. 3.
    Nirogi R, Mudigonda K, Kandikere V et al (2007) J Pharm Biomed Anal 44:379–387CrossRefGoogle Scholar
  4. 4.
    Ertu¨rk S, O¨ nal A, Cetin SM (2003) J Chromatogr B 793:193–205CrossRefGoogle Scholar
  5. 5.
    Morris MJ, Gilbert JD, Hsieh YK (1993) Biol Mass Spectrom 22:1–8CrossRefGoogle Scholar
  6. 6.
    Takano T, Abe S, Hata S (1990) Biomed Environ Mass Spectrom 19:577–581CrossRefGoogle Scholar
  7. 7.
    Zheng WH, Cai JH, Wu YL et al (2000) Fenxi Ceshi Xuebao 19:69–70Google Scholar
  8. 8.
    Stubbs RJ, Schwartz M, Bayne WF (1986) J Chromatogr 383:438–443CrossRefGoogle Scholar
  9. 9.
    Ye LY, Firby PS, Moore MJ (2000) Ther Drug Mornitoring 22:737–741CrossRefGoogle Scholar
  10. 10.
    Fu L, Dai ZS, Hou SX (2004) J Huazhong Univ Sci Technol [Med Sci] 24(2):116–119CrossRefGoogle Scholar
  11. 11.
    Neuvonen PJ, Jalava KM (1996) Clin Pharmacol Ther 60:54–61CrossRefGoogle Scholar
  12. 12.
    Choi HJ, Kim MM, Brown MS et al (1998) Yakhak Hoechi 42:473–475Google Scholar
  13. 13.
    Sun K, Wu L, Huang H et al (1998) Yaowu Fenxi Zazhi 18:165–167Google Scholar
  14. 14.
    Zhao F, Yuan Y, Shen X et al (1997) Zhongguo Yaoke Daxue Xuebao 28:288–290Google Scholar
  15. 15.
    Wu Y, Zhao J, Henion J (1997) J Mass Spectrom 32:379–387CrossRefGoogle Scholar
  16. 16.
    Davidson HM, Lukacsko P, Sun JX (2002) Clin Thers 24(1): 112–125CrossRefGoogle Scholar
  17. 17.
    Kyrklund C, Backman JT, Kivisito KT (2001) Clin Pharmacol Ther 69:340–345CrossRefGoogle Scholar
  18. 18.
    Calaf RE, Carrascal M, Gelpf E (1997) Rapid Commun Mass Spectrom 11:75–80CrossRefGoogle Scholar
  19. 19.
    Miao XS, Metcalfe CD (2003) J Chromatogr A 998:133–141CrossRefGoogle Scholar
  20. 20.
    Park EJ, Lee D, Shin YG et al (2001) J Chromatogr B 754:327–332CrossRefGoogle Scholar
  21. 21.
    Carrascal M, Schneider K, Calaf RE (1998) J Pharm Biomed Anal 17:1129–1138CrossRefGoogle Scholar
  22. 22.
    Zhu Z, Neirinck L (2003) J Chromatogr B 783:133–140CrossRefGoogle Scholar
  23. 23.
    Yu YQ, Huang JM, Weng WY (2005) Zhongguo Xinyao Yu Linchuang Zazhi 24:954–957Google Scholar
  24. 24.
    Guidance for Industry, Bioanalytical Method Validation U.S. Department of Health, Human Services, Food and Drug Administration. Center for Drug Evaluation and Research (CDER), Center for Veterinary Medicine (CVM), May 2001Google Scholar
  25. 25.
    Zhao FL, Yuan YS, Shen XY (1997) J China Pharmaceutical Univ 28(5):288–290Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2008

Authors and Affiliations

  1. 1.Department of Biopharmaceutics, School of PharmacyShenyang Pharmaceutical UniversityShenyangChina
  2. 2.Ningbo Team Pharm Co., LtdNingboChina

Personalised recommendations