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Simultaneous HPTLC Determination of Escitalopram Oxalate and Clonazepam in Combined Tablets

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Abstract

A new, simple high-performance thin-layer chromatographic method has been established and validated for simultaneous determination of escitalopram oxalate and clonazepam in a combined tablet dosage form. The drugs were separated on aluminum plates precoated with silica gel 60 F254; toluene–ethyl acetate–triethylamine 7:3.5:3 (v/v) was used as mobile phase. Quantitative analysis was performed by densitometric scanning at 258 nm. The method was validated for linearity, accuracy, precision, and robustness. The calibration plot was linear over the ranges 250–2,500 and 50–500 ng band−1 for escitalopram oxalate and clonazepam, respectively. The method was successfully applied to the analysis of drugs in a pharmaceutical formulation.

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Acknowledgments

The authors wish to express their gratitude to Cipla Ltd, Kurkumbh, India, for the sample of pure escitalopram oxalate, and to Torrent Pharmaceutical Ltd, Indrad, Gujarat, India, for the sample of pure clonazepam.

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Authors and Affiliations

  1. Department of Pharmaceutical Analysis, A.I.S.S.M.S. College of Pharmacy, Kennedy Road, Near R.T.O, Pune, 411001, India

    Nilesh Dhavale, Santosh Gandhi, Shweta Sabnis & Kailash Bothara

Authors
  1. Nilesh Dhavale
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  2. Santosh Gandhi
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  3. Shweta Sabnis
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  4. Kailash Bothara
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Corresponding author

Correspondence to Santosh Gandhi.

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Dhavale, N., Gandhi, S., Sabnis, S. et al. Simultaneous HPTLC Determination of Escitalopram Oxalate and Clonazepam in Combined Tablets. Chroma 67, 487–490 (2008). https://doi.org/10.1365/s10337-008-0524-7

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  • DOI: https://doi.org/10.1365/s10337-008-0524-7

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