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Quantitative Analysis of Triptolide in Human Whole Blood by LC–APCI-IT-MS-MS

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Abstract

In this study, the development and validation of an analytical method for triptolide in whole blood using high-performance liquid chromatography coupled with atmospheric-pressure chemical ionization ion trap tandem mass spectrometry (LC–APCI-IT-MS-MS) is reported. This is the first report of the systematic development and validation of an LC–MS-MS method for the quantitation of triptolide in human whole blood using prednisolone as an internal standard (IS). Prior to LC–MS-MS analysis, liquid–liquid extraction with ethyl acetate was used to isolate them from the biological matrix. Validation parameters such as specificity/selectivity, limit of quantitation (LOQ), linearity, precision, accuracy and stability are evaluated for this method. The calibration curve was linear (r 2 = 0.9973) in the concentration range of 0.5–100.0 ng mL−1 in human whole blood with a lower limit of quantitation of 0.5 ng mL−1. Intra- and inter-day relative standard deviations (RSDs) were less than 8.6 and 11.7%, respectively. Extraction recoveries of triptolide ranged from 81.5 to 88.1%. This assay can be used to determine trace triptolide in human whole blood.

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Acknowledgments

This work was supported by a grant from the Medicine Science Foundation of Ningbo, China (200543).

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Authors and Affiliations

  1. College of Environmental Science and Engineering, Zhejiang Gongshang University, 310035, Hangzhou, China

    Kun-bai Chen & Mei-qiang Cai

  2. Ningbo Municipal Center for Disease Control and Prevention, 315010, Ningbo, China

    Xiao-hong Chen & Mi-cong Jin

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  1. Kun-bai Chen
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  2. Mei-qiang Cai
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  3. Xiao-hong Chen
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Correspondence to Mei-qiang Cai.

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Chen, Kb., Cai, Mq., Chen, Xh. et al. Quantitative Analysis of Triptolide in Human Whole Blood by LC–APCI-IT-MS-MS. Chroma 67, 225–230 (2008). https://doi.org/10.1365/s10337-007-0503-4

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  • DOI: https://doi.org/10.1365/s10337-007-0503-4

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