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LC-MS–MS Determination of Pregabalin in Human Plasma


A rapid, sensitive and specific method to quantify pregabalin in human plasma using metaxalone as the internal standard is described. Sample preparation involved simple protein precipitation by using acetronitrile as solvent. The extract was analyzed by high-performance liquid chromatography coupled to electrospray tandem mass spectrometry (LC-MS–MS). Chromatography was performed isocratically on Thermo Hypurity C18 5 μm analytical column, (50 mm × 4.6 mm i.d.). The assay of pegabalin was linear calibration curve over the range 10.000–10000.000 ng mL−1. The lower limit of quantification was 10.000 ng mL−1 in plasma. The method was successfully applied to the bioequivalence study of pregabalin capsules (150.0 mg) administered as a single oral dose.

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  1. Wikipedia, the free encyclopedia (2007)

  2. Johannessen SI, Tomson T (2006) Clin Pharm 45(11):1061–1075

    Article  CAS  Google Scholar 

  3. LYRICA study group (2006) Int J Clin Pract 60(9):1060–1072

    Article  CAS  Google Scholar 

  4. Hindmarch I, Trick L, Ridout F (2005) Psychopharm (Berl) 83(2):133–143

    Article  CAS  Google Scholar 

  5. Pfizer Inc. FDA Approves Lyrica (Pregabalin) for Treatment of Neuropathic Pain (2006)

  6. Frampton JE, Foster RH (2005) Drugs 65(1):119–120

    Google Scholar 

  7. Freynhagen R, Strojek K, Griesing T, Whalen E, Balkenohl M (2005) Pain 115(3):254–263

    Article  CAS  Google Scholar 

  8. Fehrenbacher JC, Taylor CP, Vasko MR (2003) Pain 105(1–2):133–141

    Article  CAS  Google Scholar 

  9. Vermeij TAC, Edelbroek PM (2004) J Chromatogr B 810(2):297–303

    Article  CAS  Google Scholar 

  10. Berry D, Millington C (2005) Therapeutic Drug Monitoring 27(4):451–456

    Google Scholar 

  11. Jadhav AS, Pathare DB, Shingare MS (2007) Chromatographia 65(3–4):253–256

    Article  CAS  Google Scholar 

  12. Lovdahi MJ, Hurley TR, Tobias B (2002) J Pharm Biomed Anal 28(5):917–924

    Article  Google Scholar 

  13. Zhang Y, Pablo J, Tang D (2005) CPSA Digest, Poster Session Abstract: WP-7

  14. Sarah Osgood, Jeremy McFadden, Julie Poe, Cheryl Li, Lisa Buchholz. In: Proceedings of the 54th ASMS conference on mass spectrometry and allied topics

  15. US Department of Health and Human Services, Food and Drug Administration (2001) Guidance for industry bio analytical method validation

  16. Overholser BR, Kays MB, Sowinski KM (2003) J Chromatogr B Analyt Technol Biomed Life Sci 798(1):150–180

    Google Scholar 

  17. Liang H, Kays MB, Sowinski KM (2002) J Chromatogr B Analyt Technol Biomed Life Sci 772(1):50–68

    Google Scholar 

  18. Fernandez H, Ruperez FJ, Barbas C (2003) J Pharm Biomed Anal 31(3):450–525

    Article  CAS  Google Scholar 

  19. Radhakrishna T, Sreenivas Rao D, Om Reddy G (2002) J Pharm Biomed Anal 29(4):593–607

    Article  CAS  Google Scholar 

  20. Nemutlu E, Kir S (2003) J Pharm Biomed Anal 31(3):499–506

    Article  CAS  Google Scholar 

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Correspondence to Vikas V. Vaidya.

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Vaidya, V.V., Yetal, S.M., Roy, S.M.N. et al. LC-MS–MS Determination of Pregabalin in Human Plasma. Chroma 66, 925–928 (2007).

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