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Development of a New RAM-Chiral Column for Determination of Chiral Drugs in Biological Fluids by HPLC

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Abstract

New β-cyclodextrin (β-CD) bonded silica called RAM-Chiral has been designed for direct injection analysis of drug enantiomers in serum for liquid chromatography. The RAM-Chiral column is synthesized by two steps: introduction of a β-CD carbamate on a 70 Å pore size amino silica and coating of the external silica surface with BSA. The proteins were completely excluded and then recovered from the RAM-Chiral column. Direct injection analysis of benzodiazepin drugs in plasma can be determined on the RAM-Chiral column by using a step gradient buffered mobile phase. The recovery of racemic drugs from plasma was almost 100%.

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Authors and Affiliations

  1. Laboratoire de Chimie Organique et des Matériaux Moléculaires (CNRS-UMR 6114), Université de la Méditerranée, 163, Avenue de Luminy, 13 288, Marseille cedex 9, France

    G. Félix & D. Campèse

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  1. G. Félix
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  2. D. Campèse
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Correspondence to G. Félix.

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Félix, G., Campèse, D. Development of a New RAM-Chiral Column for Determination of Chiral Drugs in Biological Fluids by HPLC. Chroma 66, 159–164 (2007). https://doi.org/10.1365/s10337-007-0295-6

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  • DOI: https://doi.org/10.1365/s10337-007-0295-6

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