Skip to main content
SpringerLink
Log in

Simultaneous HPLC Determination of Five Flavonoids in Flos Inulae

  • Full Short Communication
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

A simple and accurate reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for simultaneous determination of five flavonoids, spinacetin, quercetin, luteolin, 6-methoxyluteolin, and isorhamnetin, in an extract of the flowers of Inula britannica L., an important Traditional Chinese Medicine (TCM). Samples were extracted with 80% ethanol. Optimum separation and detection were achieved on an ODS-3 column with a methanol–acetonitrile gradient containing 0.49% (v/v) citric acid as mobile phase. The flow rate was 1.0 mL min−1 and detection was at 360 nm. All calibration plots revealed linearity was good (r 2 = 0.999) within the concentration ranges tested. Repeatability was evaluated by performing intra-day and inter-day assays; relative standards deviations (RSD) were less than 2.8%. Recovery of the five flavonoids was between 91.5 and 103.6%, with RSD less than 6.5%. The method was successfully used for analysis of seven samples of Flos Inulae from different parts of China and was found to be simple and efficient.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Guo QL, Yang JSH (2005) Nat Prod Res Dev 17:804–809

    CAS  Google Scholar 

  2. National Commission Chinese Pharmacopoeia (2005) Pharmacopoeia of the People’s Republic of China, vol 1. Chemical Industry Press, Beijing, p 227

  3. Jiangsu New Medical College (1977) Dictionary of Traditional Chinese Materia Medica, vol 2. People’s Press, Shanghai, pp 2216–2219

    Google Scholar 

  4. Park EJ, Kim Y, Kim J (2000) Nat Prod 63:34–36

    Article  CAS  Google Scholar 

  5. Zhou BN, Bai NS, Lin LZ, Cordell GA (1994) Phytochemistry 49:157–164

    Google Scholar 

  6. Zhou BN, Bai NS, Lin LZ, Cordell GA (1993) Phytochemistry 34:249–252

    Article  CAS  Google Scholar 

  7. Wu YB, Wang YZh, Zha JP, Shi XW, Yang ShG, Zhang DQ (2006) Chin Tradit Herb Drugs 37:666–668

    Google Scholar 

  8. Shao Y, Bai NS, Zhou BN (1996) Phytochemistry 42:783–786

    Article  CAS  Google Scholar 

  9. Bai NS, Zhou Zh, Zhu NQ, Zhang L, Duan Zh, He K, Zheng YQ, Ho ChT (2005) Food Lipids 12:141–149

    Article  CAS  Google Scholar 

  10. Park EJ, Kim J (1998) Planta Med 64:752–754

    Article  CAS  Google Scholar 

  11. Liu ShX, Liu H, Yan WY, Zhang L, Bai NSh, Ho ChT (2004) Bioorg Med Chem Lett 14:1101–1104

    Article  Google Scholar 

  12. Schmid PP (1978) J Chromatogr 157:217

    Article  CAS  Google Scholar 

  13. Liu RX, Wang Q, Guo HZ, Li L, Bi KS (2005) J Pharm Biomed 39:469–476

    Article  CAS  Google Scholar 

  14. Christov R, Bankova VJ (1992) J Chromatogr 182:623

    Google Scholar 

  15. Wang SP, Huang KJ (2004) J Chromatogr A 1032:273–279

    Article  CAS  Google Scholar 

  16. Molnar-Perl I, Füzfai Zs (2005) J Chromatogr A 1073:201–227

    Article  CAS  Google Scholar 

  17. Urbanek M, Blechtova L, Pospisilova M (2002) J Chromatogr A 958:261–271

    Article  CAS  Google Scholar 

  18. Wang X, Cheng CG, Sun QL, Li FW, Liu JH (2005) J Chromatogr A 1075:127–131

    Article  CAS  Google Scholar 

  19. Zou JH, Yang JSh (2005) Chin Pharm J 40:733–736

    CAS  Google Scholar 

  20. Xu Y, Liang JY (2005) J Chin Pharm Univ 36:411–413

    CAS  Google Scholar 

  21. Wang AiG, Yin ChF, Feng XZh (2006) China J Chin Mater Med 31:694–696

    Google Scholar 

  22. Zhou R, Wang T, Du XZh (2006) China J Chin Mater Med 31:474–476

    Google Scholar 

  23. Peng JY, Fan GR, Wu YT (2006) China J Chin Mater Med 31:128–130

    CAS  Google Scholar 

  24. Jia ZS, Tang MC, Wu JM (1999) Food Chem 64:555–559

    Article  Google Scholar 

Download references

Acknowledgment

This work was supported by the Natural Science Fund of HeBei province (no. 303470).

Author information

Authors and Affiliations

  1. Department for Development of New Drugs, School of Pharmacy, Hebei Medical University, Shijiazhuang, 050017, People′s Republic of China

    Hong-Mei Geng, Di-Qun Zhang, Jian-Peng Zha & Jin-Long Qi

  2. Chemistry Department, Hengshui University, Hengshui, 053000, People′s Republic of China

    Hong-Mei Geng

Authors
  1. Hong-Mei Geng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Di-Qun Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jian-Peng Zha
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jin-Long Qi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Di-Qun Zhang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Geng, HM., Zhang, DQ., Zha, JP. et al. Simultaneous HPLC Determination of Five Flavonoids in Flos Inulae . Chroma 66, 271–275 (2007). https://doi.org/10.1365/s10337-007-0285-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-007-0285-8

Keywords

Search

Navigation