Skip to main content
SpringerLink
Log in

HPLC Quantification of Vitexine-2″-O-rhamnoside and Hyperoside in Three Crataegus Species and Their Antimicrobial and Antiviral Activities

  • Full Short Communication
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

Crataegus sp., also known as “hawthorn”, is one of the most prescribed herbal drugs and approved to be used against congestive heart failure. The extract is required to be standardized according to its flavonoid (vitexine-2″-O-rhamnoside and hyperoside) and oligomeric procyanidin content by the European Pharmacopoeia (EP). In this study, the leaves and berries of three Crataegus species including C. aronia var. aronia, C. monogyna ssp. monogyna (from two different localities) as well as C. pseudoheterophylla growing in Turkey were evaluated for their vitexine-2″-O-rhamnoside and hyperoside content by reversed-phase HPLC as well as their total procyanidin amount by a spectrophotometric method. In addition, antibacterial, antifungal, and antiviral activities of the extracts were also determined. Our results indicated that C. monogyna ssp. monogyna samples of both localities met the criteria required by the EP for their total flavonoid amount (2.49 and 2.86%) and total proanthocyanidin content (1.56 and 2.22%). According to data we obtained, the extracts have been verified to be highly effective against Candida albicans and Herpes simplex virus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Holubarsch CJF, Colucci WS, Meinertz T, Gaus W, Tendera M (2000) Eur J Heart Fail 2:431–437

    Article  CAS  Google Scholar 

  2. Zapfe G (2001) Phytomed 8:262–266

    Article  CAS  Google Scholar 

  3. Valli G, Giardina EGV (2002) J Am Coll Cardiol 39:1083–1095

    Article  Google Scholar 

  4. Degengring FH, Suter A, Weber M, Saller R (2003) Phytomed 10:363–369

    Article  Google Scholar 

  5. Schröder D, Weiser M, Klein P (2003) Eur J Heart Fail 5:319–326

    Article  Google Scholar 

  6. Kroll M, Ring C, Gaus W, Hempel B (2005) Phytomed 12:395–402

    Article  CAS  Google Scholar 

  7. Davis PH, Cullen J (1984) Crataegus In: Davis PH (ed) Flora of Turkey and the East Aegean Islands, vol.1. Edinburgh University Press, Edinburgh

  8. American Herbal PharmacopoeiaTM and Therapeutic Compendium (1999) In: Upton R (ed). AHP Press, Santa Cruz

  9. Hiermann A, Karting T, Azzam S (1986) Sci Pharm 54:331–337

    CAS  Google Scholar 

  10. National Committee for Clinical Laboratory Standards (1996), Method for broth dilution antifungal susceptibility testing yeast; approved standard, M27-A, 15, 10. NCCLS, Tucson, AZ, USA

  11. National Committee for Clinical Laboratory Standards (2002), Methods for dilution antimicrobial susceptibility tests bacteria that grow aerobically, 3rd edn, approved standard. NCCLS document M100-S12. NCCLS, Wayne, PA, USA

  12. Özçelik B, Aslan M, Orhan I, Karaoglu T (2005) Microbiol Res 160:159–164

    Article  Google Scholar 

  13. Güven K, Yücel E, Çetintaş F (2006) Pharm Biol 44:79–83

    Article  Google Scholar 

  14. Min BS, Bae KH, Kim YH, Miyashiro H, Hattori M, Shimotohno K (1999) Phytother Res 13:680–682

    Article  CAS  Google Scholar 

  15. Min BS, Jung HJ, Lee JS, Kim YH, BOK SH, Ma CM, Nakamura N, Hattori M, Bae K (1999) Planta Med 65:374–375

    Article  CAS  Google Scholar 

  16. Shahat AA, Cos P, De Bruyne T, Apers S, Hammouda FM., Ismail SI, Azzam S, Claeys M, Goovaerts E, Pieters L, Van den Berghe D, Vlietinck AJ (2002) Planta Med 68:539–541

    Article  CAS  Google Scholar 

  17. Shahat AA, Ismail SI, Hammouda FM, Azzam SA, Lemie`re G, De Bruyne T, De Swaef S, Pieters L, Vlietinck A (1998) Phytomed 5:133–136

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330, Ankara, Turkey

    I. Orhan & B. Özdeveci

  2. Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Gazi University, 06330, Ankara, Turkey

    B. Özçelik

  3. Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100, Ankara, Turkey

    M. Kartal

  4. Department of Biology, Faculty of Science and Art, Gazi University, 06500, Ankara, Turkey

    H. Duman

Authors
  1. I. Orhan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Özçelik
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Kartal
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. Özdeveci
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H. Duman
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Kartal.

Additional information

Application of Separation Techniques in Turkey.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Orhan, I., Özçelik, B., Kartal, M. et al. HPLC Quantification of Vitexine-2″-O-rhamnoside and Hyperoside in Three Crataegus Species and Their Antimicrobial and Antiviral Activities. Chroma 66 (Suppl 1), 153–157 (2007). https://doi.org/10.1365/s10337-007-0283-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-007-0283-x

Keywords

Search

Navigation