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Determination of Free Fatty Acids in Bryophyte Plants and Soil by HPLC with Fluorescence Detection and Identification by Online MS

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Abstract

A method for the determination of long and short chain free fatty acids (FFAs), using 1-[2-(p-toluenesulfonate)-ethyl]-2-phenylimidazole-[4,5-f]-9,10-phenanthrene (TSPP) as labeling reagent, has been developed. Identification of FFA derivatives was carried out by HPLC-MS with atmospheric pressure chemical ionization (APCI) in positive ion mode. Gradient elution on an Agilent Eclipse XDB-C8 column gave good separation of the derivatives. Excellent linear responses were observed and good compositional data could be obtained from as little as 200 mg of bryophyte plants and soil samples. Facile TSPP derivatization coupled with HPLC-APCI-MS analysis allowed the development of a highly sensitive method for the quantitative analysis of trace level of FFAs from biological and natural environmental samples.

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Acknowledgment

This work was supported by the Chinese Academy of Sciences (the Knowledge Innovation Program) and the National Science Foundation under Grant # 20075016.

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Authors and Affiliations

  1. Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining, 810001, People’s Republic of China

    Xianen Zhao, Honglun Wang, Jinmao You & Yourui Suo

  2. College of Chemistry Science, Qufu Normal University, Qufu, Shandong, 273165, People’s Republic of China

    Jinmao You

  3. Graduate school of Chinese Academy of Sciences, Beijing, 100039, People’s Republic of China

    Xianen Zhao

Authors
  1. Xianen Zhao
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  2. Honglun Wang
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  3. Jinmao You
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  4. Yourui Suo
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Correspondence to Yourui Suo.

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Zhao, X., Wang, H., You, J. et al. Determination of Free Fatty Acids in Bryophyte Plants and Soil by HPLC with Fluorescence Detection and Identification by Online MS. Chroma 66, 197–206 (2007). https://doi.org/10.1365/s10337-007-0271-1

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  • DOI: https://doi.org/10.1365/s10337-007-0271-1

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