Abstract
A novel, simple and rapid stability-indicating high-performance liquid chromatographic (HPLC) method for pravastatin sodium (PRA) was successfully developed and validated for the assay of in tablets. Chromatographic separation was achieved isocratically on a C18 column (150 mm × 4.6 mm) utilizing a mobile phase of methanol-phosphate buffer (pH 7; 0.02 M) (57:43, v/v) at a flow rate of 1.0 mL min−1 with UV detection at 238 nm. A linear response (r = 0.9999) was observed in the range of 1–5 µg mL−1. The method showed good recoveries (100.50%) and the relative standard deviation of intra and inter-day were 1.40%. The method can be used for both quality control assay of pravastatin in tablets and for stability studies as the method separates pravastatin from its degradation products and tablet excipients.
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An erratum to this article can be found at http://dx.doi.org/10.1365/s10337-006-0063-z
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Önal, A., Sagirli, O. Development of a Selective LC Method for the Determination of Pravastatin Sodium. Chroma 64, 1–6 (2006). https://doi.org/10.1365/s10337-006-0843-5
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DOI: https://doi.org/10.1365/s10337-006-0843-5