Skip to main content
SpringerLink
Log in

Determination of Gatifloxacin in Semen by HPLC with Diode-Array and Fluorescence Detection

  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

The objective of this work was to develop an analytical HPLC method, using DAD and fluorescence detection, for determination of gatifloxacin in semen. A reversed-phase column was used with 90:10 water-acetonitrile, containing 10 mM TBA and 25 mM citric acid, as mobile phase. Semen was deproteinized with acetonitrile. Recovery was 95 ± 10%. The limits of quantification by DAD and fluorescence were 2.3 and 0.03 μg.mL−1 respectively, with RSD of 3.4% for DAD and 2.8% for fluorescence. The method with fluorescence detection was used for quantification of gatifloxacin in the semen of patients under treatment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Borner K, Hartwig H, Lode H (2000) Chromatographia Supplement 51:S105–S107

    Google Scholar 

  • Liang HK, Michael B, Sowinski KM (2002) J Chromatogr B 772:53–63

    Article  CAS  Google Scholar 

  • Overholser BR, Kays MB, Sowinski KM (2003) J Chromatogr B 798:167–173

    Article  CAS  Google Scholar 

  • Vishwanathan K, Bartlett MG, Stewart JT (2001) Rapid Commun Mass Spectrom 15:915–919

    Article  CAS  Google Scholar 

  • Lian N, Sun CY, Zhao HC (2002) Fenxi Ceshi Xuebao 21:79–81 (in Chinese)

    CAS  Google Scholar 

  • Colunga-González LY, Gallegos-de Lerma MG, Waksman-de Torres N, Salazar-Cavazos ML (2005) Anal Lett 38:2355–2364

    Article  Google Scholar 

  • Salazar ML, López-de Alba PL, Piñeyro-López A, Waksman-de Torres N (2002) J Liq Chromatogr Related Technol 25:1747–1760

    Article  CAS  Google Scholar 

  • Bidlingmeyer BA (1992) Practical HPLC Methodology and Applications. John Wiley and Sons, USA

Download references

Author information

Authors and Affiliations

  1. Departamento de Química Analítica, U.A.N.L. Facultad de Medicina, Madero y Aguirre Pequeño Col. Mitras Centro C.P, 64460, Mty, N.L, México

    M. L. Salazar Cavazos, L. Y. Colunga González & N. Waksman de Torres

  2. Departamento de Patología, U.A.N.L. Facultad de Medicina, Madero y Aguirre Pequeño Col. Mitras Centro C.P, 64460, Mty, N.L, México

    G. Gallegos de Lerma

Authors
  1. M. L. Salazar Cavazos
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Y. Colunga González
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Gallegos de Lerma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. Waksman de Torres
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to N. Waksman de Torres.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cavazos, M.L.S., González, L.Y.C., Lerma, G.G.d. et al. Determination of Gatifloxacin in Semen by HPLC with Diode-Array and Fluorescence Detection. Chroma 63, 605–608 (2006). https://doi.org/10.1365/s10337-006-0807-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-006-0807-9

Keywords

Search

Navigation