Abstract
A novel HPLC method is described for separation of the three stereoisomers of octahydroindole-2-carboxylic acid (Oic), an intermediate in the synthesis of perindopril. The chiral mobile phase contained the complex of Cu(II) with the optically active selector L-phenylalaninamide (L-PheA), and an ion-pair reagent, sodium 1-octanesulfonate. The effects of the concentrations of the Cu(II)–L-PheA complex and the ion-pair reagent, mobile phase pH, ionic strength, acetonitrile content, and column temperature were studied. Satisfactory resolution was achieved for three stereoisomers, RRR-, SSS-, and SRR-Oic.
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Sun, S., Jia, Y., Zeng, N. et al. Chiral Ligand-Exchange Chromatography for Separation of Three Stereoisomers of Octahydroindole-2-carboxylic Acid. Chroma 63, 331–335 (2006). https://doi.org/10.1365/s10337-006-0752-7
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DOI: https://doi.org/10.1365/s10337-006-0752-7