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Development and Validation of Improved Reversed Phase-HPLC Method for Simultaneous Determination of Curcumin, Demethoxycurcumin and Bis-Demethoxycurcumin

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Abstract

Isocratic reversed phase high performance liquid chromatographic (HPLC) method using RP C18 column was developed for simultaneous determination of the curcuminoids. Mobile phase consisted of acetonitrile:0.1% trifluro-acetic acid (50:50) and flow rate was 1.5 mL min−1 and elution was monitored at 420 nm. Validation in selected conditions showed that the chosen method is sensitive, selective, precise and reproducible with linear response of detector for the simultaneous determination of curcumin (C), demethoxycurcumin (DMC) and bis-demethoxycurcumin (BDMC). The limits of detection were 27.99, 31.91 and 21.81 ng mL−1 for C, DMC and BDMC, respectively. Limits of quantitation for C, DMC and BDMC, were 84.84, 96.72 and 66.10 ng mL−1, respectively. Linear range was form 100 to 600 ng mL−1. The mean ± SD percent recoveries of curcuminoids were 99.87 ± 0.34, 100.09 ± 0.48 and 100.10 ± 0.60% of C, DMC and BDMC, respectively. Further, the method was used for quantitation of curcuminoids from turmeric rhizome.

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Acknowledgement

Authors are thankful to UGC, New Delhi, India for providing financial assistance. Authors are also thankful to Fluka, Switzerland; Dr. Ann Li, National University of Taiwan, Taiwan; Sami Chemicals, Bangalore, India (Sabinsa Corporation, USA) for providing gift sample of curcuminoids, C, DMC and BDMC, respectively.

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Authors and Affiliations

  1. Department of Pharmaceutics, Bharati Vidyapeeth University, Poona College of Pharmacy, Erandawane, Pune, 411 038, Maharashtra, India

    B.-K. Jadhav & A.-R. Paradkar

  2. Department of Pharmaceutical Chemistry, Bharati Vidyapeeth University, Poona College of Pharmacy, Erandawane, Pune, 411 038, Maharashtra, India

    K.-R. Mahadik

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  1. B.-K. Jadhav
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  2. K.-R. Mahadik
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  3. A.-R. Paradkar
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Correspondence to A.-R. Paradkar.

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Jadhav, BK., Mahadik, KR. & Paradkar, AR. Development and Validation of Improved Reversed Phase-HPLC Method for Simultaneous Determination of Curcumin, Demethoxycurcumin and Bis-Demethoxycurcumin. Chroma 65, 483–488 (2007). https://doi.org/10.1365/s10337-006-0164-8

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  • DOI: https://doi.org/10.1365/s10337-006-0164-8

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