Advertisement

Chromatographia

, Volume 64, Issue 9–10, pp 509–515 | Cite as

Simple Extraction and HPLC Determination of UV-A and UV-B Filters in Sunscreen Products

  • D. De Orsi
  • G. Giannini
  • L. Gagliardi
  • R. Porrà
  • S. Berri
  • A. Bolasco
  • I. Carpani
  • D. TonelliEmail author
Original

Abstract

The determination of 11 organic UV filters, worldwide authorised in sunscreen formulations, was performed by HPLC with UV spectrophotometric detection. The filters determined were: phenylbenzimidazole sulfonic acid, benzophenone-4, benzophenone-3, 4-methylbenzylidene camphor, ethylhexyl methoxycinnamate, octocrylene, methylene bis-benzotriazolyl tetramethylbutyl phenol, butyl methoxydibenzoylmethane, bis-ethylhexyloxyphenol methoxyphenyltriazine, ethylhexyl triazone, and diethylamino hydroxybenzoyl hexyl benzoate. Three analytical methods were developed which were based on an ultrasonic extraction of the sample in methanol/1% aqueous acetic acid (70:30, v/v) or dimethylacetamide/propan-2-ol (1:1, v/v) in dependence of the water or fat-solubility of the filter, followed by HPLC separation and quantitation. HPLC was carried out using three different columns and eluting conditions. Standard calibration curves were linear for all the analytes over the concentration range 0.5–100 μg mL−1. UV measurements were carried out at 280, 300, 310 or 360 nm with the aid of a diode array detector in dependence on the maximum absorption of each filter. Quantitative recoveries were obtained for all sunscreen compounds determined in homemade formulations. The proposed analytical procedure has been successfully applied for the analysis of commercial samples in order to check the presence of UV filters and to determine their concentration.

Keywords

Column liquid chromatography Quality control UV filters in cosmetic products Sunscreen products 

Notes

Acknowledgments

The Authors wish to thank Mr Gianluigi Pinci from Istituto Superiore di Sanità for his helpful support in computer science.

References

  1. 1.
    Diffey BL (1991) Phys Med Biol 36:299–328CrossRefGoogle Scholar
  2. 2.
    De Gruijl FR, van Kranen HJ, Mullenders LHF (2001) J Photochem Photobiol B 63:19–27CrossRefGoogle Scholar
  3. 3.
    Gasparro FP, Mitchnick M, Nash JF (1998) Phochem Photobiol 68:243–256CrossRefGoogle Scholar
  4. 4.
    Ferguson J (1997) European guidelines (COLIPA) for evaluation of sun protection factors. In: Lowe NJ, Shaath NA, Pathak MA (eds) Sunscreens: development, evaluation, and regulatory aspects. Marcel Dekker, New York, pp 513–525Google Scholar
  5. 5.
    Gilchrest BA, Soter NA, Hawk JL, Barr RM, Black AK, Hensby CN, Mallet AI, Greaves MW, Parrish JA (1983) J Am Acad Dermatol 9:213–219CrossRefGoogle Scholar
  6. 6.
    Takeuchi T, Uitto J, Bernstein EF (1998) J Invest Dermatol 110:343–347CrossRefGoogle Scholar
  7. 7.
    Menter JM, Patta AM, Sayre RM, Dowdy J, Willis I (2001) Photodermatol Photoimmunol Photomed 17:114–120CrossRefGoogle Scholar
  8. 8.
    Osterwalder U, Luther H, Herzog B (2001) SOFW J Verlag Chem Ind H Ziolkowsky 127:45, 50, 54Google Scholar
  9. 9.
    Schmidt T, Ring J, Abeck D (1998) Dermatology 196:354–357CrossRefGoogle Scholar
  10. 10.
    European Directive 76/768/EEC and its successive amendments, basic act 31976L0768Google Scholar
  11. 11.
    FDA, Department of Health, Human Services 21CFR Parts 310, 352, 700 and 740, RIN 0910-AA01 (1999) Sunscreen Drug Products for over-the-counter human Use Final Monograph, Federal Register, Rules and Regulations, 64:27666–27693Google Scholar
  12. 12.
    Japanese Standard of Cosmetic Ingredients (1985) Yakuji Nippo Ltd., TokyoGoogle Scholar
  13. 13.
    Salvador A, Chisvert A (2005) Anal Chim Acta 537:1–14CrossRefGoogle Scholar
  14. 14.
    Chisvert A, Salvador A (2002) J Chromatogr A 977:277–280CrossRefGoogle Scholar
  15. 15.
    Ro KW, Choi JB, Lee MH, Kim JW (1994) J Chromatogr A 688:375–382CrossRefGoogle Scholar
  16. 16.
    Rastogi SC, Jensen GH (1998) J Chromatogr A 828:311–316CrossRefGoogle Scholar
  17. 17.
    Schakel DJ, Kalsbeek D, Boer K (2004) J Chromatogr A 1049:127–130CrossRefGoogle Scholar
  18. 18.
    Salvador A, Chisvert A (2005) Anal Chim Acta 537:15–24CrossRefGoogle Scholar
  19. 19.
    Dolan JW (2004) LC-GC Eur 17:624–628Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2006

Authors and Affiliations

  • D. De Orsi
    • 1
  • G. Giannini
    • 1
  • L. Gagliardi
    • 1
  • R. Porrà
    • 1
  • S. Berri
    • 1
  • A. Bolasco
    • 2
  • I. Carpani
    • 3
  • D. Tonelli
    • 3
    Email author
  1. 1.Dipartimento del FarmacoIstituto Superiore di SanitàRomeItaly
  2. 2.Dipartimento di Studi di Chimica e Tecnologia delle Sostanze Biologicamente AttiveUniversità di Roma 1RomeItaly
  3. 3.Dipartimento di Chimica Fisica ed InorganicaBolognaItaly

Personalised recommendations