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Simultaneous Determination of Acteoside, Astragaloside IV and Icariside-I in the Traditional Chinese Medicinal Preparation Shenbao by HPLC–MS

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Abstract

A liquid chromatography–electrospray (ES)–mass spectrometric method for the simultaneous determination of Acteoside, Astragaloside IV and Icariside-I in the Traditional Chinese Medicinal Preparation Shenbao tablets is described. The samples were separated on an Alltima C18 column by linear gradient elution using water–acetonitrile as the mobile phase. Some operational parameters of the ESI interface were optimized. The method is linear over the range of 0.1–10μg mL−1 for Acteoside, Astragaloside IV, and 0.03–3μg mL−1 for Icariside-I. The method has a precision (%CV) of <20%, and an accuracy (%RE) of < ± 10%. It can be used as a complementary method for quality control of Shenbao Tablets while HPLC–UV can be used for the other main components (Icariin, Icariside-II, Psoralen, Isopsoralen, and Osthol).

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Acknowledgments

This research was supported by Hi-Tech Research and Development Program of China, Ministry of Science and Technology of the People’s Republic of China (No: 2002AA2Z3214).

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Authors and Affiliations

  1. China Pharmaceutical University, Nanjing, 210009, China

    Ying Tu, Luhua Zhao, Lin Zhu, Guangji Wang & Bingren Xiang

Authors
  1. Ying Tu
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  2. Luhua Zhao
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  3. Lin Zhu
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  4. Guangji Wang
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  5. Bingren Xiang
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Correspondence to Luhua Zhao.

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Tu, Y., Zhao, L., Zhu, L. et al. Simultaneous Determination of Acteoside, Astragaloside IV and Icariside-I in the Traditional Chinese Medicinal Preparation Shenbao by HPLC–MS. Chroma 64, 453–458 (2006). https://doi.org/10.1365/s10337-006-0053-1

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  • DOI: https://doi.org/10.1365/s10337-006-0053-1

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