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Monitoring of Mycophenolic Acid in the Plasma of Transplant Patients by Capillary Electrophoresis

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Abstract

Plasma samples from 60 transplant patients on mycophenolate mofetil therapy were analysed using validated capillary electrophoresis (CE) method to determine mycophenolic acid (MPA) and its main metabolite mycophenolic acid glucuronide. Enzyme multiplied immunoassay technique (EMIT) values were available for the same samples. The differences between the results from the two methods was clarified by using Bland–Altman analysis and further statistical analysis. More than 90% of the results showed a positive bias, with EMIT giving higher levels than CE.

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Acknowledgments

S. Ünsalan was supported by a grant from DAAD (Deutscher Akademischer Austauschdienst).

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Authors and Affiliations

  1. Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Marmara University, Tıbbiye Street No. 49, 34668, Haydarpasa-Istanbul, Turkey

    S. Ünsalan

  2. Universitätsklinikum Münster, Klinik und Poliklinik für Kinder- und Jugendmedizin, - Pädiatrische Hämatologie und Onkologie, Munster, Germany

    G. Hempel, G. Würthwein & J. Boos

  3. Institut für Pharmazeutische und Medizinische Chemie, Westfälische Wilhelms-Universität Münster, Munster, Germany

    G. Hempel

  4. Institut für Klinische Chemie und Laboratoriumsmedizin, Munster, Germany

    M. Fobker

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  1. S. Ünsalan
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  2. G. Hempel
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  3. M. Fobker
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  4. G. Würthwein
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  5. J. Boos
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Correspondence to S. Ünsalan.

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Ünsalan, S., Hempel, G., Fobker, M. et al. Monitoring of Mycophenolic Acid in the Plasma of Transplant Patients by Capillary Electrophoresis. Chroma 64, 419–421 (2006). https://doi.org/10.1365/s10337-006-0046-0

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  • DOI: https://doi.org/10.1365/s10337-006-0046-0

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