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Detection of Stanozolol and Its Major Metabolites in Human Urine by Liquid Chromatography-Tandem Mass Spectrometry

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Abstract

The determination of stanozolol and its metabolites in human urine has been of particular interest in sports drug testing due to its frequently revealed misuse. A simple and rapid sample preparation procedure based on consecutive solid-phase and liquid–liquid extraction with subsequent re-extraction followed by liquid chromatography and electrospray ionization tandem mass spectrometry was established. It allowed the determination of stanozolol and its metabolic products 16β-OH-stanozolol and 4β-OH-stanozolol in human urine at detection limits of 0.1, 0.2 and 0.2 ng mL−1, respectively, with recoveries ranging from 5 to 38%. The robust nature of the assay and the efficient removal of interfering biological matrix provides excellent signal-to-noise ratios, and, thus, a rapid alternative to established procedures utilizing multiple solid-phase extraction or immunoaffinity chromatography strategies. More than 15 doping control urine specimens tested positive for stanozolol during the last 12 months have been confirmed using the described approach.

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Acknowledgments

The authors thank the Manfred-Donike Gesellschaft, Cologne, for support. This project was carried out with support of the International Bureau of the Federal Ministry of Education and Research, Bonn, and the Manfred-Donike Gesellschaft, Cologne.

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Authors and Affiliations

  1. Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Carl-Diem Weg 6, 50933, Cologne, Germany

    M. Thevis, G. Fußhöller, H. Geyer, U. Mareck, G. Sigmund, A. Koch, A. Thomas & W. Schänzer

  2. Moscow Anti-Doping Centre, Elizavetinsky per. 10, 105005, Moscow, Russia

    G. Rodchenkov

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  1. M. Thevis
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  2. G. Fußhöller
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  3. H. Geyer
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  4. G. Rodchenkov
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  5. U. Mareck
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  6. G. Sigmund
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  7. A. Koch
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  8. A. Thomas
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  9. W. Schänzer
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Correspondence to M. Thevis.

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Thevis, M., Fußhöller, G., Geyer, H. et al. Detection of Stanozolol and Its Major Metabolites in Human Urine by Liquid Chromatography-Tandem Mass Spectrometry. Chroma 64, 441–446 (2006). https://doi.org/10.1365/s10337-006-0043-3

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  • DOI: https://doi.org/10.1365/s10337-006-0043-3

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