Skip to main content
SpringerLink
Log in

LC Determination of Anethole in Aniseed Drinks

  • Short Communication
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

A method for the determination of anethole in aniseed drinks by high performance liquid chromatography (HPLC) is proposed. A C-18 column and a methanol-water (80:20, v/v) mobile phase with isocratic elution were used. UV detection at 257 nm was carried out. Validation was performed according to the EURACHEM guidelines, considering precision, trueness, robustness, linearity and sensitivity. The detection and quantification limits were 2.3 and 7.7 μg L−1, respectively. Recoveries of 95–101% were obtained. Repeatability, calculated as relative standard deviation was 0.9%. Reproducibility under intermediate precision conditions, expressed as relative standard deviation, was 1.6%. The method was compared with the gas chromatographic one, proposed by the European regulations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. González MJ, Martínez MC, Aguilar MV (1988) Z Lebens Unters Forsch 187:325–329

    Article  Google Scholar 

  2. Andrea V, Nadia N, Teresa RM, Andrea A (2003) J Agric Food Chem 51:4978–4983

    Article  CAS  Google Scholar 

  3. Diéguez SC, Peña MLG, Gómez EF (2003) J Agric Food Chem 51:7385–7390

    Article  Google Scholar 

  4. Directive (CEE) No 1576/89. Off J Eur Commun L160 (12–6–1989)

  5. Besharati-Seidani A, Jabbari A, Yamini Y (2005) Anal Chim Acta 530:155–161

    Article  CAS  Google Scholar 

  6. Rodrigues VM, Rosa PTV, Marques MOM, Petenate AJ, Meireles MAA (2003) J Agric Food Chem 51:1518–1523

    Article  CAS  Google Scholar 

  7. Regulation (EC) No 2091/2002. Off J Eur Commun L322 (26–11–2002)

  8. Brereton P, Hasnip S, Bertrand A, Wittkowski C, Guillou C (2003) Trends Analyt Chem 22:19–25

    Article  CAS  Google Scholar 

  9. Snyder LR, Kirkland JJ, Glajch JL (1997) Practical HPLC method development. Wiley, New York

  10. EURACHEM (1998) The fitness for purpose of analytical method: a laboratory guide to method validation and related topics, http://www.eurachem.ul.pt

  11. Maroto A, Boqué R, Riu J, Rius FX (2001) Anal Chim Acta 440:171–184

    Article  CAS  Google Scholar 

  12. González AG, Herrador MA, Asuero AG (1999) Talanta 48:729–736

    Article  Google Scholar 

  13. Miller JN, Miller JC (2000) Statistics and chemometrics for analytical chemistry, 4th edn. Prentice-Hall, London

  14. Passing H, Bablock JA (1983) J Clin Chem Biochem 21:709–720

    CAS  Google Scholar 

  15. Youden WJ (1967) Statistical techniques for collaborative test. AOAC, Washington

  16. González AG, Herrador MA, Asuero AG (2005) Talanta 65:1022–1030

    Article  Google Scholar 

  17. Placket RL, Burmann JP (1946) Biometrica 33:305–325

    Article  Google Scholar 

  18. Huber L (1998) LC-GC Int 11:96–105

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Chemistry, University of Seville, 41012, Seville, Spain

    J. M. Jurado, A. Alcázar, F. Pablos & M. J. Martín

Authors
  1. J. M. Jurado
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Alcázar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. Pablos
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. J. Martín
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to F. Pablos.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jurado, J.M., Alcázar, A., Pablos, F. et al. LC Determination of Anethole in Aniseed Drinks. Chroma 64, 223–226 (2006). https://doi.org/10.1365/s10337-006-0001-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-006-0001-0

Keywords

Search

Navigation