Abstract
The enantiomers of some amino acids have been separated on commercial chiral TLC plates in reversed-phase mode. The effect of the pH* of the aqueous-organic mobile phase on the retention and mobility of the enantiomers and on selectivity was investigated. It was shown that for most of the amino acids investigated the highest enantioselectivity was obtained at pH* 3–4 or 6–7. The drift and disturbance of the baseline on the chromatograms were also much smaller at pH* 3–4 and 6–7.
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References
Davankov VA (1988) In: Davankov VA, Navratil JD, Walton HF (eds) Ligand exchange chromatography. CRC Press, Boca Raton, FL, USA, pp 67–175
Davankov VA, Rogozin SV (1971) J Chromatogr 60:280–283
Günther K (1988) J Chromatogr 448:11–30
Veigl E, Lindner W (1994) J Chromatogr A 660:255–268
Grobuschek N, Schmid MG, Tuscher C, Ivanova M, Gübitz G (2002) J Pharm Biomed Anal 27:599–605
Miyazawa T, Minowa H, Imagawa K, Yamada T (2004) Chromatographia 60:45–50
Nikoliæ N, Veselinoviæ D, Vuèina J, Lingeman H, Karljikoviæ-Rajiæ K (2003) J Pharm Biomed Anal 32:1159–1166
Huyn MH, Han ChS, Lee ChW, Lee YK (2002) J Chromatogr A 950:55–63
Günther K, Möller K (2003) In: Sherma J, Fried B (eds) Handbook of thin-layer chromatography, 3rd edn. Marcel Dekker, New York, pp 471–535
Nyiredy Sz, Dallenbach-Toelke K, Sticher O (1988) J Chromatogr 450:241–252
Nyiredy Sz (2002) J Planar Chromatogr 15:454–457
Siouffi A-M, Piras P, Roussel Ch (2005) J Planar Chromatogr 18:5–12
Polak B, Gołkiewicz W (1996) Chem Anal (Warsaw) 41:931–937
Chai Z, Xu S, Hou X (2004) Chromatographia 60:25–31
Gołkiewicz W, Polak B (1994) J Planar Chromatogr 6:453–457
Lepri L, Del Bubba M, Cincinelli A (2003) In: Nyiredy Sz (ed) Planar chromatography. A retrospective view for the third millennium. Springer, Research Institute for Medicinal Plants, Budakalasz, Hungary, pp 516–549
Bushan R, Martens J (2003) In: Sherma J, Fried B (eds) Handbook of thin-layer chromatography, 3rd edn. Marcel Dekker, New York, pp 389–425
Dzido TH, Soczewiñski E (1990) J Chromatogr 516:461–466
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Polak, B., Gołkiewicz, W. & Tuzimski, T. Effect of Mobile Phase pH* on Chromatographic Behaviour in Chiral Ligand-Exchange Thin-Layer Chromatography (CLETLC) of Amino Acid Enantiomers. Chroma 63, 197–201 (2006). https://doi.org/10.1365/s10337-005-0681-x
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DOI: https://doi.org/10.1365/s10337-005-0681-x