Skip to main content
SpringerLink
Log in

RP-TLC Determination of S(+) and R(−) Ibuprofen in Drugs With the Application of Chiral Mobile Phase and UV Densitometric Detection

  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

A chromatographic-densitometric method for the identification and determination of S(+) and R(−) ibuprofen has been developed. The chromatographic separation was carried out on RP-TLC plates and the following mobile phase was used: β-cyklodextrin methanol (15 +1, v/v). The UV densitometric detection was carried out at λ=222 nm. The method is of high sensitivity, the limit of detection for S(+) and R(−) ibuprofen is 1 μg mg; of good precision and repeatability, the obtained results are within the range x mean ±2S; of high recovery for both isomers approximately 99.18% and wide range of linearity from 0.01% to 0.3%. The presence of both isomers S(+) and R(−)ibuprofen was observed in all preparations at comparable concentrations from 56.04% to 66.16% for S(+) isomer and from 33.84% to 43.99% for R(−) isomer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Pharmindex (2004) MediMedia Int Sp z oo Warszawa

  • Perrin C, Matthijs N, Mangelings D, Granier-Layaux C, Maftouh M, Massart DL, Vander Heyden Y (2002) J Chromatogr A 966:119–134

    Article  CAS  Google Scholar 

  • Loftsson T, Masson M (2001) Int J Pharm 225:15–30

    Article  CAS  Google Scholar 

  • Yuchun X, Huizhou L, Jiayong Ch (2000) Int J Pharm 196:21–26

    Article  CAS  Google Scholar 

  • Van Overbeke A, Baeyens W, Dewaele C (1995) J Liqiud Chromatogr 18(12):2427–2443

    Google Scholar 

  • Asami M, Nakamura KI (1995) Chirality 7:28–33

    Article  CAS  ISI  Google Scholar 

  • Van Overbeke A, Baeyens W, Van Den Bossche W, Dewaele C (1994) J Pharm Biomed Anal 12(7):901–909

    Google Scholar 

  • Hyun MH, Cho SM, Ryoo JJ, Kim MS (1994) J Liquid Chromatogr 17(2):317–328

    CAS  Google Scholar 

  • Haginaka J, Murashima T, Seyama Ch (1994) J Chromatogr A 677:229–237

    Article  CAS  Google Scholar 

  • Karger AE, Stoll E, Hänsel W (1994) Pharmazie 49:155–159

    CAS  ISI  Google Scholar 

  • Fanali S (1996) J Chromatogr A 735:77–121

    Article  CAS  Google Scholar 

  • Pawełczyk E, Hermann T, Podstawy trwałości leków (1982) PZWL Warszawa

  • Q2B: Validation of Analytical Procedures (1996) ICH Steering Committee

  • Zajac M, Pawełczyk E, Chemia leków (2000) AM Poznań

Download references

Author information

Authors and Affiliations

  1. Department of Inorganic and Analytical Chemistry, Collegium Medicum, Jagiellonian University, 9 Medyczna Str, 30-688, Kraków, Poland

    J. Krzek, M. Starek & D. Jelonkiewicz

Authors
  1. J. Krzek
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Starek
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. Jelonkiewicz
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. Krzek.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Krzek, J., Starek, M. & Jelonkiewicz, D. RP-TLC Determination of S(+) and R(−) Ibuprofen in Drugs With the Application of Chiral Mobile Phase and UV Densitometric Detection. Chroma 62, 653–657 (2005). https://doi.org/10.1365/s10337-005-0677-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-005-0677-6

Keywords

Search

Navigation