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LC-ESI-MS Determination of Bilobalide and Ginkgolides in Canine Plasma

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Abstract

A sensitive and selective method using liquid chromatography with electrospray ionization mass spectrometric detection was developed for the quantification of bilobalide and ginkgolides in canine plasma. The analytes were extracted with diethyl ether-dichloromethane-isopropanol (6:3:1, v/v) after spiking the samples with daidzein (internal standard). The lower limit of quantification (LLOQ) of the method was 2.5 µg L−1 for ginkgolide B and 10.0 µg L−1 for bilabolide, ginkgolide A and ginkgolide C. The accuracy of the method was within 15% of the actual values over a wide range of plasma concentrations. The intra-day and inter-day precision was better than 15% (R.S.D.). Finally, the LC-ESI-MS method was successfully applied to study the pharmacokinetics of ginkgolides and bilabolide after administration of Ginkgo biloba extracts to dogs.

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Authors and Affiliations

  1. Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China

    Jingling Tang, Jin Sun, Yinghua Sun, Fude Cui  & Zhonggui He

Authors
  1. Jingling Tang
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  2. Jin Sun
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  3. Yinghua Sun
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  4. Fude Cui
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  5. Zhonggui He
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Correspondence to Zhonggui He.

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Tang, J., Sun, J., Sun, Y. et al. LC-ESI-MS Determination of Bilobalide and Ginkgolides in Canine Plasma. Chroma 63, 53–58 (2006). https://doi.org/10.1365/s10337-005-0671-z

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  • DOI: https://doi.org/10.1365/s10337-005-0671-z

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