Abstract
A reversed phase high performance liquid chromatography method was established for the simultaneous determination of eight major constituents, namely gallic acid, paeoniflorin sulfonate, catechin, albiflorin, paeoniflorin, benzoic acid, pentagalloylglucose and benzoylpaeoniflorin in red and white peony roots, the two commonly used traditional Chinese medicinal herbs. The optimal conditions of separation and detection were achieved on a C18 analytical column with a gradient mobile phase consisting of acetonitrile and 0.015% phosphoric acid at the flow rate of 1.0 mL min−1 and detection wavelength set at 230 nm. All calibration curves showed good linear regression (r>0.9995) within test ranges. This method provided good reproducibility with overall intra-and inter-day precision of less than 5% and 4% and good accuracy with recovery of more than 93%, respectively. The method was successfully applied to determine 71 samples of red and white peony roots collected from different areas. The results indicated that the contents of eight compounds varied significantly among the samples determined, which mainly resulted from processing procedure and habitat variation. The roots of Paeonia veitchii Lynch. contained a much higher amount of gallic acid and pentagalloylglucose than that of Paeonia lactiflora Pall., which could be used to distinguish the two similar species.
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References
National Commission of Chinese Pharmacopoeia (2005) Phamacopoeia of the People's Republic of China. Chemical Industry Press, Beijing, pp69–109
Yin J, Guo L G (1994) Modern research and clinical application of Chinese materia medica (I). Beijing Xueyuan Press, Beijing pp 247–356
Tabata K, Matsumoto K, Watanabe H (2000) Jpn J Pharmacol 83:25–30
Satoh K, Nagai F, Ushiyama K, Yasuda I, Seto T, Kano I (1997) Biochem Pharmacol 53:611–614
Okubo T, Nagai F, Seto T, Satoh K, Ushiyama K, Kano I (2000) Biol Pharm Bull 23:199–203
Ruan JL, Zhao ZX, Zeng QZ, Qian ZM (2003) Chin Pharmacol Bull 19:965–970
Liu YX, Ma YZ (1995) Chin Trad Herb Drugs 26:437–440
Yoshizaki M, Tomimori T, Yoshioka S, Namba T (1977) Yakugaku Zasshi 97:916–912
Ikeda N, Fukuda T, Jyo H, Shimada Y, Murakami N (1996) Yakugaku Zasshi 116:138–147
Wu HK, Sheu SJ (1996) J Chromatogr A 753:139–146
Wang CL, Chuang WC, Sheu SJ (1996) J High Resol Chromatogr 19:530–533
Lu Z, Morinaga O, Tanaka H, Shoyama Y (2003) Biol Pharm Bull 26:862–866
Zhang KR, Liu RX, Xu JB and Bi KS (2003) Chin Pharm J 38:793–79
Suto K, Masuda M, Maruta T, Sagara K, Mizutani T(2003) Shoyakugaku Zasshi 46:9–13
Zhou HT, Luo YQ, Hu SL, Li RK, Liu HW, Feng XF (2003) Chin Pharm J 38:654–657
Hayes PY, Lehmann R, Penman K, Kitching W, De Voss JJ (2005) Tetrahedron lett 46:2615–2618
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Wang, Q., Liu, R., Guo, H. et al. Simultaneous LC Determination of Major Constituents in Red and White Peony Root. Chroma 62, 581–588 (2005). https://doi.org/10.1365/s10337-005-0660-2
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DOI: https://doi.org/10.1365/s10337-005-0660-2