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Determination of Carvedilol and its Impurities in Pharmaceuticals

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Abstract

A reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for separation of carvedilol and its impurities from Karvileks tablets. The best separation was achieved on a 100 mm × 4.6 mm, 5 µm particle size, Chromolit RP 8e column. Use of acetonitrile-water, 45:55 (v/v), adjusted to pH 2.5 with formic acid, as mobile phase at a flow rate of 0.5 mL min−1 enabled acceptable resolution of carvedilol, in large excess, from possible impurities, in a short elution time. UV detection was performed at 280 nm. Linearity, accuracy, precision, selectivity, and robustness were validated and found to be satisfactory. Overall, the proposed method was found to be highly sensitive, suitable, and accurate for quantitative determination of carvedilol and its impurities in dosage forms and in raw materials.

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References

  • Abshangen U (1987) J Cardiovasc Pharmacol 10 (Suppl. 11):23

  • Nakamura K, Kusano K, Nakamura Y, Kakishita M, Ohta K, Nagase S, Yamamoto M, Miyaji K, Saito H, Morita H, Emori T, Matsubara H, Toyokuni S, Ohe T (2002) Circulation 105:2867–2871

    CAS  Google Scholar 

  • Kukin LM, Kalman J, Charney HR, Levy KD, Buchholz-Varley C, Ocampo NO, Eng C (1999) Circulation 99:2645–2651

    CAS  Google Scholar 

  • Packer M, Fowler BM, Roecker BE, Coats JSA, Katus AH, Krum H, Mohacsi P, Rouleau LJ, Tendera M, Staiger C, Holcslaw LT, Amann-Zalan I, DeMets LD (2002) Circulation 106(22):2194–2199

    Google Scholar 

  • Ruffolo RR, Boyle DA, Brooks DP, Feuerstein GZ, Venuti RP, Lukas MA, Poste G (1992) Cardiovasc Drug Rev 10:127–157

    CAS  Google Scholar 

  • Poole-Wilson PA, Swedberg K, Cleland JG, Di Lenarda A, Hanrath P, Komajda M, Lubsen J, Lutiger B, Metra M, Remme WJ, Torp-Pedersen C, Scherhag A, Skene A (2002) Eur J Heart Fail 4:321–329

    CAS  Google Scholar 

  • Behn F, Laer S, Scholz H (2001) Chromatographia 53(11/12):641–644

    Google Scholar 

  • Behn F, Laer S, Scholz H (2001) J Chromatogr Sci 39(3):121–124

    CAS  Google Scholar 

  • Hokama N, Hobara N, Kameya H, Ohshiro S, Sakanashi M (1999) J Chromatogr B 732(1):233–238

    CAS  Google Scholar 

  • Machida M, Watanabe M, Takechi S, Kakinoki S, Nomura A (2003) J Chromatogr B 798(2):187–191

    Article  CAS  Google Scholar 

  • Ptacek P, Macek J, Klima J (2003) J Chromatogr B 789(2):405–410

    CAS  Google Scholar 

  • Lamprecht G, Stoschitzky K (2004) Chromatographia 59(9/10):551–554

  • Yang E, Wang S, Kratz J, Cyronak MJ (2004) J Pharm Biomed Anal 36(3):609–615

    Article  CAS  Google Scholar 

  • Clohs L, McErlane KM (2003) J Pharm Biomed Anal 31(3):407–412

    Article  CAS  Google Scholar 

  • Oravcova J, Sojkova D, Lindner W (1996) J Chromatogr B 682(2):349–357

    CAS  Google Scholar 

  • Phuong NT, Lee BJ, Choi JK, Kang JS, Kwon K (2004) Arch Pharmacol Res 27(9):973–977

    CAS  Google Scholar 

  • Li XX, Ni H, Li YM, Huai YW (2005) Spectrochim Acta A 61(5):855–859

    Google Scholar 

  • Radi A, Elmogy T (2005) Il Farmaco 60(1):43–46

    Article  CAS  Google Scholar 

  • Caron G, Steyaert G, Pagliara A, Reymond F, Crivori P, Gaillard P, Carrupt P, Avdeef A, Comer J, Box JK, Girault HH, Testa B (1999) Helv Chim Acta (82):1211–1222

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Correspondence to J. Stojanović.

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Stojanović, J., Marinković, V., Vladimirov, S. et al. Determination of Carvedilol and its Impurities in Pharmaceuticals. Chroma 62, 539–542 (2005). https://doi.org/10.1365/s10337-005-0656-y

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  • DOI: https://doi.org/10.1365/s10337-005-0656-y

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