Advertisement

Chromatographia

, Volume 62, Issue 5–6, pp 225–231 | Cite as

Determination of Florfenicol in Freshwater, Sediments and Bryophyte Fontinalis antipyretica by HPLC with Fluorescence Detection

  • H. Pouliquen
  • M. L. Morvan
Article

Abstract

Novel procedures for the determination of florfenicol in freshwater, sediments and bryophyte Fontinalis antipyretica, using reversed-phase high-performance liquid chromatography are described. Liquid chromatography was performed on a 5 µm PuroSpher RP-18E® column using methanol and 0.05 M phosphate buffer (18/82 v/v, pH 7.3) as mobile phase (0.8 ml min−1) and fluorescence detection (excitation wavelength 265 nm and emission wavelength 295 nm). Florfenicol was determined in centrifuged freshwater samples. Florfenicol was extracted from sediments and bryophytes samples by using a solid-liquid extraction step followed by a solid phase extraction step. Linearity was confirmed over the concentration range 25–1000 ng mL−1 water and 50-1000 ng g−1 sediment or bryophyte. Limits of detection and quantitation were 8 and 25 ng mL−1 water and 17 and 50 ng g−1 sediment or bryophyte respectively. Mean extraction recoveries of florfenicol from sediments and bryophyte were from 85.9 to 109.1%.

Keywords

Column liquid chromatography Florfenicol Freshwater and sediment samples Fontinalis antipyretica 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ho SP, Hsu TY, Chen MH, Wang WS (2000) J Vet Med Sci 62(5):479–485CrossRefPubMedGoogle Scholar
  2. Martinsen B, Horsberg TE, Varma KJ, Sams R (1993) Aquaculture 112:1–11CrossRefGoogle Scholar
  3. Delépée R, Pouliquen H, Le Bris H (2004) Sci Total Environ 322:243–253CrossRefPubMedGoogle Scholar
  4. Nagata T (1995) Chemical analysis of chloramphenicols. In: Okaé H, Nakazawa H, Harada K, Macneil JD (eds) Chemical analysis of antibiotics in agriculture. AOAC International, Arlington, VA, USA, pp. 207–234Google Scholar
  5. Nagata T, Oka H (1996) J. Agric Food Chem 44(5):1280–1284CrossRefGoogle Scholar
  6. Hormazabal V, Steffenak I, Yndestad M (1993) J Chromatogr B 616(1):161–165Google Scholar
  7. De Craene BA, Deprez P, D’Haese E, Nelis HJ, Van den Bossche W, De Leenheer AP (1997) Antimicrob Agents Chemother 41(9):1991–1995PubMedGoogle Scholar
  8. Vue C, Schmidt LJ, Stehly GR, Gingerich WH (2002) J Chromatogr B 780:111–117CrossRefGoogle Scholar
  9. Stamper MA, Papich MG, Lewbart GA, May SB, Plummer DD, Stoskopf MK (2003) J Zoo Wildlife Med. 34(1):3–8Google Scholar
  10. Hillaert S, Van den Bossche W (2004) J Pharm Biomed Anal 36(3):437–440CrossRefPubMedGoogle Scholar
  11. Hormazabal V, Steffenak I, Yndestad M (1996) J Chromatogr A 724:364–366CrossRefGoogle Scholar
  12. Cooper AD, Tarbin JA, Farrington WHH, Shearer G (1998) Food Addit Contam 15(6):637–644PubMedGoogle Scholar
  13. Caniou I, Nikolaides E, Tsoukali H, Stratis JA, Zachariadis GA (1995) J Liq Chromatogr Related Technol 18(17):3519–3527Google Scholar
  14. Roudaut B (1996) J Liq Chromatogr Related Technol 19(7):1097–1105Google Scholar
  15. Ramos M, Reuvers T, Aranda A, Gomez J (1994) J Liq Chromatogr 17(2):385–401Google Scholar
  16. Delépée R, Pouliquen H (2002) J Chromatogr B 775:89–95CrossRefGoogle Scholar
  17. Delépée R, Pouliquen H (2003) Anal Chim Acta 475:117–123CrossRefGoogle Scholar
  18. Di Pietra AM, Piazza V, Andrisano V, Cavrini V (1995) J Liq Chromatogr Related Technol 18(17):3529–3543Google Scholar
  19. Pouliquen H, Le Bris H, Pinault L (1994b) Quim Anal (Barcelona), 13(Suppl 1):S109–S113Google Scholar
  20. Chapuzet E, Mercier N (1997) S.T.P. Pharma Pratiques 7:169–194Google Scholar
  21. Lang JR, Bolton S (1991) J Pharm Biomed Anal 9:435–442CrossRefPubMedGoogle Scholar
  22. Pouliquen H, Le Bris H, Pinault L (1992) Mar Ecol Progr Ser 89:93–98Google Scholar
  23. Pouliquen H, Le Bris H, Pinault L (1994a) J Liq Chromatogr 17(4):929–945Google Scholar

Copyright information

© Friedr. Vieweg & Sohn/GWV Fachverlage GmbH 2005

Authors and Affiliations

  1. 1.Unité Mixte de Recherche INRA-ENVN de Chimiothérapie Aquacole et Environnement, Ecole Nationale Vétérinaire de NantesNantes cedex 03France

Personalised recommendations