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Determination of Diazepam in Human Plasma by Solid-Phase Microextraction and Capillary Gas Chromatography-Mass Spectrometry

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Abstract

A simple, sensitive, and reproducible solid-phase microextraction and capillary gas chromatography-mass spectrometry (SPME-GC-MS) method for determination of diazepam in human plasma is described. The optimum conditions for the SPME procedure were as following: direct extraction mode with a polydimethylsiloxane (PDMS) fiber (100 μm film thickness), 250 μL of sample plasma matrix modified with a solution containing sodium chloride (10% weight by volume) and 4.25 mL of a phosphate buffer solution (0.1mol L−1, pH 6.9), extraction temperature 55°C under a magnetic stirring rate of 2500 rpm for 30 min, followed by the drug thermal desorption (250°C) in a GC injection port for 10 min. The limit of quantification of diazepam in plasma was 10.0 ng mL−1, with a coefficient of variation lower than 14.0% and linearity from 10.0 to 1000.0 ng mL−1, which allows diazepam analyses from sub to therapeutic levels.

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Authors and Affiliations

  1. Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, 13560-970, Brasil

    M. H. De. Oliveira & F. M. Lancas

  2. Departamento de Química, Faculdade de Filosofia Ciěncias e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, 14040-901, SP, Brasil

    M. E. C. Queiroz

  3. Curso de Ciências Farmacêuticas, Universidade de Ribeirão Preto, Ribeirão Preto, SP, 14096-380, Brasil

    D. Carvalho & S. M. Silva

Authors
  1. M. H. De. Oliveira
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  2. M. E. C. Queiroz
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  3. D. Carvalho
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  4. S. M. Silva
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  5. F. M. Lancas
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Corresponding author

Correspondence to F. M. Lancas.

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Oliveira, M.H.D., Queiroz, M.E.C., Carvalho, D. et al. Determination of Diazepam in Human Plasma by Solid-Phase Microextraction and Capillary Gas Chromatography-Mass Spectrometry. Chroma 62, 215–219 (2005). https://doi.org/10.1365/s10337-005-0601-0

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  • DOI: https://doi.org/10.1365/s10337-005-0601-0

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