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High-Performance Liquid Chromatographic Analysis of Neurotoxin β-N-Oxalyl-α, β-diaminopropionic Acid (β-ODAP), Its Non-neurotoxic Isomer α-ODAP and Other Free Amino Acids in Lathyrus sativus

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Abstract

A simple method is proposed for the simultaneous analysis of the neurotoxin β-N-oxalyl-α, β-diaminopropionic acid (β-ODAP) and its isomer α-ODAP, as well as other free amino acids, in Lathyrus sativus extract based on reversed-phase (C18 column) high-performance liquid chromatography (HPLC) of their para-nitro-benzyloxycarbonyl chloride (PNZ-Cl) derivatives. Samples were derivatized in 0.5 M NaHCO3 within 2 min at ambient temperature by adding 50 mM PNZ-Cl in acetonitrile. The PNZ-derivatives were quantified by their UV-absorption at 260 nm. Detection limits were approximately 8 ng mL−1 for both α-, β-isomers, 9 ng mL−1 for homoarginine at a signal-to-noise ratio of 3:1. The coefficients of determination were 0.9967–0.9991. The method was validated by the determination of β-, α-ODAP and homoarginine contents in ethanol-water (30:70, v/v) extracts from the seed and foliage of Lathyrus sativus.

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Acknowledgments.

This work was supported by NKBRSF Project (G2000018603) and the National Natural Science Foundation of China (No. 30270965). Dr. Peter S. Spencer who is from Oregon Health and Science University, Portland, USA, is thanked for assistance with the manuscript.

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Authors and Affiliations

  1. State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, 730000, China

    Zeyi Yan, Yongmin Liang & Zhixiao Li

  2. School of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, 730070, China

    Yanpeng Wang

  3. School of Life Science, Lanzhou University, Lanzhou, 730000, China

    Chengjin Jiao & Fengmin Li

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  1. Zeyi Yan
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  2. Yanpeng Wang
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  3. Chengjin Jiao
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  4. Fengmin Li
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  5. Yongmin Liang
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  6. Zhixiao Li
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Correspondence to Zhixiao Li.

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Yan, Z., Wang, Y., Jiao, C. et al. High-Performance Liquid Chromatographic Analysis of Neurotoxin β-N-Oxalyl-α, β-diaminopropionic Acid (β-ODAP), Its Non-neurotoxic Isomer α-ODAP and Other Free Amino Acids in Lathyrus sativus. Chroma 61, 231–236 (2005). https://doi.org/10.1365/s10337-005-0500-4

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