Abstract
Capillary zone electrophoresis has been used for the determination of gatifloxacin from its pharmaceutical preparation (tablets), using fused silica capillary. Separation was performed after hydrodynamic injection; the separation was achieved by applying 21 Kv voltage. Phosphate buffer solution (pH 9.5) was used as separation electrolyte. Detection was at 280 nm using a UV- detector. Under these experimental conditions the analysis takes 8 min. A linearity range for gatifloxacin was between 20.0 μg mL−1 to 60.0 μg mL−1. The method was validated and was found to be specific, precise, accurate, reproducible and robust and can be applied for the routine analysis of gatifloxacin from formulation and bulk drug.
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References
Baker DR (1995) Capillary electrophoresis. John Wiley and sons, New York
Shah VP et al (1991) European Journal of drug metabolism and pharmacokinetics 16:11–19
Bolton S (1997) Pharmaceutical Statistics Practical And Clinical Applications. Marcel Dekker, USA
Spriet A, Beiler D (1987) European Journal Of Drug Metabolism And Pharmacokinetics pp 109–112
Overholser BR, Kays MB, Sowinski KM (2003) J Chromatogr B Analyt Technol Biomed Life Sci 798(1):167–173
Liang H, Kays MB, Sowinski KM (2002) J Chromatogr B Analyt Technol Biomed Life Sci 772(1):53–63
Dubreuil L, Behra-Miellet J, Neut C, Calvet L (2003) Clin Microbiol Infect 9(11):1133–1138
Blondeau JM, Laskowski R, Bjarnason J, Stewart C (2000) Int J Antimicrob Agents 14(1):45–50
Bassetti M, Dembry LM, Farrel PA, Callan DA, Andriole VT (2001) Diagn Microbiol Infect Dis 41(3):143–148
Kaale E, Van Schepdael A, Roets E, Hoogmartens J (2001) J Chromatogr A 924(1–2):451–458
Radhakrishna T, Sreenivas Rao D, Om Reddy G (2002) J Pharm Biomed Anal 29(4):593–607
Saenz-Lopez R, Fernandez-Zurbano P, Tena MT (2003) J Chromatogr A 990(1–2):247–258
Nemutlu E, Kir S (2003) J Pharm Biomed Anal 31(2):393–400
Fernandez H, Ruperez FJ, Barbas C (2003) J Pharm Biomed Anal 31(3):499–506
Navalon A, Araujo L, Prieto A, Vilchez JL (2002) J Chromatogr B Analyt Technol Biomed Life Sci 772(1):65–72
Hou YH, Wu CY, Ding WH (2002) J Chromatogr A 976(1–2):207–213
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Sane, R., Menon, S., Pathak, A. et al. Capillary Electrophoretic Determination of Gatifloxacin from Pharmaceutical Preparation. Chroma 61, 303–306 (2005). https://doi.org/10.1365/s10337-005-0496-9
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DOI: https://doi.org/10.1365/s10337-005-0496-9