Skip to main content
SpringerLink
Log in

Determination of Homoisoflavonoids in Ophiopogon japonicus by RP-HPLC

  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

The homoisoflavonoid content of 14 samples of Ophiopogon japonicus collected from different parts of China have been investigated. Three principal homoisoflavonoids, methylophiopogonanone A, methylophiopogonanone B, and 6-aldehydo-isoophiopogonanone A, were analyzed simultaneously by reversed-phase high-performance liquid chromatography with a binary mixture of acetonitrile and 0.3% aqueous acetic acid as mobile phase. The recovery of the method was 98.5–102.6% and good linearity (r > 0.9990) was obtained for all the homoisoflavonoids over a relatively wide concentration range. The results indicated that the homoisoflavonoid content of Ophiopogon japonicus varied significantly from species to species, and from locality to locality. The quality and identity of Ophiopogon japonicus from the herb market can be determined from the homoisoflavonoid content.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Chang HM, But PP (1986) In: Pharmacology and application of Chinese Materia Medica. World Scientific, Philadelphia, p 543

  • National Commission of Chinese Pharmacopoeia (2000) Pharmacopoeia of the People’s Republic of China. Chemical Industry Press, Beijing, p 253

  • Amschler G, Frahm AM, Hatzelmann A, Kilian U, Muller-Doblis D, Muller-Doblis U (1996) Planta Med 62:534–539

    Google Scholar 

  • Asano T, Murayama T, Hirai Y, Shoji J (1993) Chem Pharm Bull 41:391–393

    Google Scholar 

  • Chang JM, Shen CC, Huang YL, Chien MY, Ou JC, Shieh BJ, Chen CC (2002) J Nat Prod 65:1731–1733

    Google Scholar 

  • Anh NTH, Sung TV, Porzel A, Franke K, Wessjohann LA (2003) Phytochemistry 62:1153–158

    Google Scholar 

  • Zhu Y, Yan K, Tu G (1987) Phytochemistry 26:2873–2874

    Google Scholar 

  • Watanabe Y, Sanada S, Ida Y, Shoji J (1985) Chem Pharm Bull 33:5358–5363

    Google Scholar 

  • Tada A, Kasai R, Saitoh T, Shoji J (1980) Chem Pharm Bull 28:1477–1484

    Google Scholar 

  • Zhu Y, Yan K, Tu G (1988) J Chromatogr 437:265–267

    Google Scholar 

  • Escarpa A, Gonzalez MC (2000) Chromatographia 51:37–43

    Google Scholar 

  • Yu BY, Xu GJ (1995) Trad Chin Med 26(4):205–210

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 201203, P.R. China

    Guan Ye & Chenggang Huang

  2. The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100083, P.R. China

    Min Ye & Dean Guo

Authors
  1. Guan Ye
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Min Ye
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dean Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chenggang Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chenggang Huang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ye, G., Ye, M., Guo, D. et al. Determination of Homoisoflavonoids in Ophiopogon japonicus by RP-HPLC. Chroma 61, 121–125 (2005). https://doi.org/10.1365/s10337-004-0486-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-004-0486-3

Keywords

Search

Navigation