A simple, reliable and reproducible method was developed for determination of psoralen and isopsoralen in several traditional Chinese medicines, such as Shouwu Pian, Gubenkechuan Pian, was described in this paper. It was based on capillary zone electrophoresis with amperometric detection (CZE-AD). A carbon-disc electrode was used as working electrode. The optimal conditions of separation and detection were pH7.6 phosphate buffer (20 mM), 20 kV for the separation voltage and 1.00V (vs. Ag/AgCl) for the detection potential. The linear ranges were 6.90 × 10-5~1.30 × 10-7 mol L-1for psoralen and 5.0 × 10-5~1.80 × 10-7 mol·L-1 for isopsoralen with the correlation coefficient of 0.9984 and 0.9994, respectively. The detection limits for psoralen and isopsoralen were 4.2 × 10-8 mol·L-1 and 6.0 × 10-8 mol·L-1 based on the signal to noise ratio of 3. The method built in this paper was directly applied to the determination of several traditional Chinese medicines with some simple pretreatment. The assay result was satisfactory.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Wu H (1978) Communication of Chinese Traditional Medicine 9:32–35
Mariano TM, Vetrano AM (2002) Biochem Pharmacol 63:31–39
Bethea D, Fullmer B, Syed S, Tiano J, Rischko C, Gillespie L (1999) J Dermatol Sci 19:78–88
Abdel Naser MB, Wollina U, El Okby M, El Shiemy S (2004) Clin Exp Dermatol 29:380–382
Edelson R, Berger C, Gasparro F (1987) N Engl J Med 316:297–303
Jarvenpaa EP, Jestoi MN, Huopalahti R (1997) Phytochem Anal 8:250–256
Makki S, Muret P, Said AM, Bassignot P, Humbert P, Agache P, Millet J (1996) Int J Pharm 133:245–252
Jorge Llano, Johan Raber, Leif A Eriksson (2003) J Photochemistry and Photobiology A: Chemistry 154:235–243
Gunther EJ, Yeasky TM, Gasparro FP, Glazer PM (1995) Cancer Res 55:1283–1288
Declan P Bogan, Richard O’Kennedy (1996) J Chromatography B Biomed Sci Appl 686:267–273
Okamura N, Miki H, Orii H (1999) J Pharm Biomed Anal 19:603–612
Daenens P, Van Boven M (1971) J Chromatogr A 57:319–321
Mignot B, Guillaume Y, Makki S, Murret E, Cavalli E, Truong TT, Thomassin M, Guinchard C (1997) J Chromatogr B Biomed Sci Appl 700:283–285
Cao X, Tian Y, Zhang T, Li X, Ito Y (1999) J Chromatogr A 855:709–713
Cardoso CAL, Vilegas W, Honda NK (2000) J Pharm Biomed Anal 22:203–214
Chen G, Zhang H, Ye JN (2000) Anal Chim Acta 423:69–76
Chen G, Zhang J, Ye JN (2001) Anal J Chromatogr A 923:255–262
Fang YZ, Fang XM, Ye JN (1995) Chem J Chin Univ 10:1514–1518
Fu CG, Song LN, Fang YZ (1999) Anal Chim Acta 399:259–263
Ching-Erh Lin, Chung-Chuan Hsueh, Wei-Chen Lin, Chia-Chieh Chang (1996) J Chromatogr A 746:295–299
About this article
Cite this article
Wu, F., Wang, A., Zhou, Y. et al. Determination of Psoralen and Isopsoralen in Traditional Chinese Medicines by Capillary Zone Electrophoresis with Amperometric Detection. Chroma 61, 157–160 (2005). https://doi.org/10.1365/s10337-004-0476-5