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Off-Line Concentration of Bisphenol A and Three Alkylphenols by SPE then On-Line Concentration and Rapid Separation by Reverse-Migration Micellar Electrokinetic Chromatography

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Abstract

A method for separation of four environmentally important compounds (three alkylphenols and bisphenol A) by cyclodextrin-modified reverse-migration micellar electrokinetic chromatography (CD-RM-MEKC) has been optimized. On-line concentration, stacking using reverse-migrating micelles and a water plug (SRW), was used to improve the limit of detection. Two main factors affecting the efficiency of enhancement by SRW, injection sample length and water-plug length, are discussed in detail. Compared with the normal 2-s injection, more than 121-fold enhancement of peak area and more than 71-fold enhancement of peak height were achieved with 120-s sample injection after 120-s water-plug injection. With this optimized separation method and SRW technology, limits of detection were found to be 0.089, 0.030, 0.0091, and 0.033 mg L−1 for 4-nonylphenol, 4-tert-octylphenol, bisphenol A, and 4-tert-butylphenol, respectively. Calibration plots (R2 ≥ 0.9968) and relative standard deviations (RSD, %) of corrected peak areas and migration time were also satisfactory. Combined with off-line concentration by solid-phase extraction (SPE) this method might be used to determine these contaminants at ppb levels in groundwater samples.

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Authors and Affiliations

  1. Research Center for Eco-Environmental Sciences, The Chinese Academy of Sciences, P.O. Box 2871, Beijing, 100085, P.R. China

    Xinghong Li, Shaogang Chu, Shan Fu, Lingling MA, Xiufen Liu & Xiaobai Xu

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  1. Xinghong Li
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  2. Shaogang Chu
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  3. Shan Fu
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  4. Lingling MA
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  5. Xiufen Liu
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  6. Xiaobai Xu
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Correspondence to Xiaobai Xu.

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Li, X., Chu, S., Fu, S. et al. Off-Line Concentration of Bisphenol A and Three Alkylphenols by SPE then On-Line Concentration and Rapid Separation by Reverse-Migration Micellar Electrokinetic Chromatography. Chroma 61, 161–166 (2005). https://doi.org/10.1365/s10337-004-0471-x

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  • DOI: https://doi.org/10.1365/s10337-004-0471-x

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